IPI-2I<Porcine ileal epithelial cells>

&Porcine ileal epithelial cells& c=r> [Product number] c=r>
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Cell
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(Porcine ileal epithelial cells)
[Background information] c=r>
See cell instructions
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Main source of cells B The amount is %. If the cell status is poor, you can increase the B amount to %. After the cells are stable, adjust the B amount back to %. For first-time experiments, in general cell culture, double antibodies need to be added to prevent cell contamination and cell confluence. When % is reached, we start shipping, so as to maintain good cell viability when the cells are received. Precautions for resuscitating cells:
Change the medium on the same day after receiving the cells. Replace all cells with the customer's own fresh culture medium, put them into the incubator, and digest them the next day.
Observe while digesting. According to the shape of the cells, stop the digestion time and pipe the edges of the cells at half-minute intervals. If the cells can be piped down, the digestion will be terminated. Customers explore for better digestion time.
There is a cell operation manual accompanying the cells, please read it carefully.
Remember to add % double resistance to reduce the probability of contamination. In addition, freeze the seeds as soon as possible for later use.
The after-sales time is one week, which is limited to quality problems of the cells themselves. Cell problems caused by Party B's own improper operations (irregular operation, over-digestion, contamination caused by not adding double-antibodies, etc.) are not included in the after-sales scope.
After receiving the cells, if the cells are in poor condition or encounter problems during culture, please contact us as soon as possible on the same day for processing. Timely feedback on the cell status and cell photos on the same day is an important reference for after-sales tracking and processing, so please pay attention to it.
When the cells are just received, fetal bovine serum can be cultured with % for two or three days, which will help the cells recover as quickly as possible. After the cell status is stable, the % can be adjusted back.
(Among them, the strips are for adherent cells. For details of suspended cells, please refer to the cell operating instructions)& c=r>
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Resuscitation culture bottle (one bottle ) or cryopreservation tubes (two)
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*
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Pig
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Ileum
[Cell morphology] c=r>
See the cell instructions section for details
[Cell Characteristics] c=r>
Adhesion
& Selection and use of cell culture medium:
: Simple ingredients, preferred for adherent cells.
: Divided into high sugar type and low sugar type.
: Suitable for cells with low cell density and difficult growth. Such as screening of hybrid cells and screening and culturing of transformed cells after transfection.
One of the most widely used culture media. The first choice for suspension cell culture, mainly for lymphocytes, but also suitable for other cells.
, culture medium: complete ingredients, good culture effect.
Medium: Suitable for mouse and human diploid cell culture.
Medium: Suitable for single cell isolation and culture and serum-free culture.
&rMedium: Especially suitable for the growth of primary cells and cells that are difficult to culture. & c=r>
% (c)
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Cells do not contain mycoplasma, bacteria, yeast and fungi
[Cultivation conditions] c= r>
See cell instructions for details
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Recommendation: Change the medium once every two days
[Freezing conditions] c=r>
For details, please refer to the cell instructions
[Main literature] c=r>
Please refer to relevant published articles for details
Summary of frequently asked questions in cell culture experiments:
Generally, after receiving cells, customers should What to pay attention to? After receiving the cells, the customer does not open the lid, and then places them in the incubator for a few hours (depending on the cell density) to observe the growth of the cells under an inverted microscope and take pictures of the cells at different times (it is recommended to observe the culture after receiving the cells) The color of the base and whether there is any leakage, take pictures of the cells under a microscope, one photo each), and exclude the contamination of the cells themselves. The cells are received unopened. If there is contamination, we will be responsible for sending a free cell line.
If there is no abnormality when receiving the cells, please observe the cell density under the microscope for "porcine ileal epithelial cells". If they are adherent cells and the confluence does not exceed %, aspirate the culture medium from the culture bottle and leave If the culture medium continues to culture and exceeds % confluence, please subculture according to cell culture conditions. For suspended cells, aspirate the culture medium, centrifuge at 10 rpm for 10 minutes, aspirate the supernatant, resuspend the cells at the bottom of the tube with fresh culture medium, and then move them back to the culture bottle. It is recommended to use preparation B for cell digestion solution. Use solution preparation with caution.
.How long will it take for the express cells to arrive? Should I send frozen cells or resuscitated cells?
We use express delivery. Generally, if you are out of town, please confirm the local temperature before sending cells. If the temperature is lower than 10 degrees Celsius, we will send frozen cells by mail.
.Can I use a medium that is different from the original culture conditions? cannot. Each cell line has its own specific and adapted cell culture medium. If a culture medium that is different from the original culture conditions is suddenly used, most cells will not be able to adapt immediately, resulting in the cells being unable to survive.
.Can I use a serum type that is different from the original culture conditions? cannot. Serum is an extremely important source of nutrients for cell culture, so the type and quality of serum will have a great impact on cell growth. Serum from different species differs in the amount or content of some substances or molecules. Wrong use of serum often results in cells being unable to survive.
.What is the appropriate proportion of serum in the culture medium? Serum is an extremely important source of nutrients for cell culture, so the content of serum will have a great impact on cell growth. The content of serum is generally %%, and should not be lower than % or higher than %, because if the content is too low, it will lead to insufficient cell nutrition, and if it is too high, it will destroy the osmotic pressure balance. It is generally recommended that the serum content is %, which can be added or subtracted appropriately.
When should the culture medium be replaced? Depending on the cell growth density, or according to the replacement time in the basic data of the cell line, the culture medium can be replaced on time. You can also replace it based on the color change of the indicator.
Why do the purchased recovery cells fall off? Because there will inevitably be vibrations during transportation, it can be collected by centrifugation after receipt.
Why are there too few cells in the purchased cell cryovials after thawing? When researchers culture frozen cells, they find that the number of cells is too low. This is mostly due to errors in the centrifugation process, which results in physical damage to the cells and cell loss. It is recommended not to centrifuge immediately after thawing the cells, but to wait until the cells grow overnight before changing the culture medium.
What are the possible reasons for purchased cell death or poor cell viability? When researchers experience poor survival rates in cell culture, common reasons can be summarized as: using the wrong culture medium or poor quality culture media. The wrong serum was used or the serum was of poor quality. Unfreeze process error. After thawing the frozen cells, the cells are washed and centrifuged. Suspension cells are mistaken for dead cells. Wrong culture temperature was used. Cells were left in &&C for too long.
What’s the reason why the frozen vial I received was cracked, the cap cracked, or the cap fell off? If the cryovial bottle cap is cracked or the bottle body is broken, it may be because the operator used improper force when clamping the cryovial tube, causing the cryovial tube to crack. It is recommended to use hemostatic forceps to clamp it carefully. In addition, the loose cap of the cryovial tube is due to the physical phenomenon of thermal expansion and contraction. The cryovial tube may cause cell contamination. Therefore, the cryovial tube should be re-opened immediately when putting it into and taking out the liquid nitrogen barrel. Tighten once.
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, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell line organisms , growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture services. Technical support makes your experiments hassle-free!
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, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cells strain organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide cell culture Comprehensive technical support makes your experiments hassle-free!
&Porcine ileal epithelial cells& c=r>
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The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks. , providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can Provide comprehensive technical support for cell culture so that your experiments will be hassle-free!
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, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell line organisms , growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture services. Technical support makes your experiments hassle-free!
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, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cells strain organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide cell culture Comprehensive technical support makes your experiments hassle-free!
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, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell line biology body, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture Technical support makes your experiments hassle-free!
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c=r>
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell line biology body, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture Technical support makes your experiments hassle-free!
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, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing Cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide cells Develop all-round technical support so that your experiments will be hassle-free!
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c=r>
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell line biology body, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture Technical support makes your experiments hassle-free!
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c=r>
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell line biology body, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture Technical support makes your experiments hassle-free!