EFM-19; human breast cancer cells

Human breast cancer cells

[Product number]

[Chinese abbreviation]
Cells

[Chinese name]
Human Breast cancer cells

[Background information]
See cell instructions

[Product source]
The main sources of cells are B and a few well-known universities at home and abroad.

The company provides cell characteristics such as adherent, semi-adherent, suspended, semi-suspended, adherent, and suspension mixed. Generally, the B amount of cell culture is 0. If there is a bad cell condition, the B amount can be increased until the cells are stable. After that, adjust the amount B back. For first-time experimenters, in general cell culture, double antibodies need to be added to prevent cell contamination. When the cell confluence is reached, we start sending human breast cancer cells
This way, the cells can be maintained when they are received. , good cell viability. Precautions for resuscitating cells
Change the medium on the same day after receiving the cells. Replace all cells with the customer's own fresh culture medium, put them in the incubator, and digest them the next day.
Observe while digesting. According to the shape of the cells, stop the digestion time and pipe the edges of the cells at half-minute intervals. If the cells can be piped down, the digestion will be terminated. Customers explore for better digestion time.
There is a cell operation manual accompanying the cells, please read it carefully.
Remember to add double-antibodies to reduce the probability of contamination. In addition, freeze the seeds as soon as possible for later use.
The after-sales time is one week, which is limited to quality problems of the cells themselves. Cell problems caused by Party B’s own improper operation, excessive digestion, contamination caused by not adding double-antibodies, etc. are not included in the after-sales scope.
After receiving the cells, if the cells are in poor condition or encounter problems during culture, please contact us as soon as possible on the same day for processing. Timely feedback on the cell status and cell photos on the same day is an important reference for after-sales tracking and processing, so please pay attention to it.
When the cells are first received, fetal bovine serum can be cultured for two or three days to help the cells recover as quickly as possible. After the cells are stabilized, they can be adjusted back.
Among them, the strips are for adherent cells. For details about suspended cells, please see the cell operating instructions

[Product packaging]
One recovery culture bottle or two cryopreservation tubes

[Product Specifications]
*

[Security Level]

[Product Type]
People

[Tissue source]
Breast

[Cell morphology]
Epithelial cell-like

[Cell characteristics]
Patch Wall cell culture medium

Selection and use of culture medium for adherent cells
It has simple ingredients and is the first choice for adherent cells.
Divided into high sugar type and low sugar type.
Suitable for cells with low cell density and difficult growth. Such as screening of hybrid cells and screening and culturing of transformed cells after transfection.
One of the most widely used culture media. The first choice for suspension cell culture, mainly for lymphocytes, but also suitable for other cells.
The culture medium for human breast cancer cells
has complete ingredients and good culture effect.
The culture medium is suitable for mouse and human diploid cell culture.
The culture medium is suitable for single cell isolation and culture and serum-free culture.
The culture medium is particularly suitable for the growth of primary cells and cells that are difficult to culture.
bbr B

[Cell detection]
The cells do not contain Mycoplasma B bacteria, yeast and fungi

[Cultivation conditions]
See cells for details Instructions

[Passaging method]
It is recommended to change the medium every two days

[Freezing conditions]
See the cell instructions for details

[Main literature]
Please refer to relevant published articles for details
Operation procedures and precautions after receiving cells
If the cells are adherent cells and are suspended or partially suspended when received status, please centrifuge the suspended cells immediately, add serum-based complete medium to a new culture dish/bottle and continue culturing for another day. At the same time, replace the remaining adherent cells in the original culture bottle with serum-based complete medium for culture for another day. After 3 days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem
Adherent cells grow slowly and the serum concentration should be increased appropriately and cannot exceed the maximum, or it can be based on The growth density of the cells should be considered after trypsin digestion and then transferred to a new culture flask to continue culturing
If the adherent cells grow unevenly and grow into islands, the cells can be digested, resuspended and dispersed. Cells, add fresh culture medium for culture
Please strictly follow aseptic operations for routine cell culture and passage procedures
Aspirate the culture medium in the original culture bottle, rinse the cells twice with B buffer, and add trypsin for digestion Pay attention to the digestion time of cells, usually control the digestion situation under the microscope. When the cell edge shrinks and the adhesion is loose, it is not recommended to digest until the cells float. Remove the trypsin, add complete culture medium, gently pipet the cell layer, and try to remove the trypsin. Blow off the cell layer and disperse it
Transfer part of the cell suspension to a new culture dish/bottle, add appropriate complete culture medium, mix the cell suspension evenly, and culture it in an incubator
Human breast cancer cells
Pay attention to the changes in culture medium value, change the medium regularly every week, and repeat the operation or freeze it after the cell density reaches the level
Pay special attention to if you are using a public laboratory or are new to cell culture, It is recommended to add double antibody culture
After receiving the cells, please replace it with fresh culture medium containing serum as soon as possible. If you need to continue to use the original bottle due to special circumstances, please add additional serum to the original bottle of culture medium. The longest time of continued use should not exceed an hour
Do not digest the adherent cells immediately on the day they are received. Please change the medium of the cells and place them in an incubator to incubate until the next day before digestion and passage. Please give priority to the diameter of the culture dish for passage. Culture
If the wall of the culture bottle is broken or leaks when signing for it, please take a photo record in time and contact the laboratory

The company will provide background information. Growth characteristics of the organism, source organ type, morphology culture Conditional application refers to the recovery and cryopreservation instructions information such as disease tissue cryopreservation conditions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!

B
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, human breast cancer cells
The company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instruction information, We have rich experience in cell line culture and can provide all-round technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!

/
, the company provides background information human breast cancer cells
organism growth characteristics source organ type morphology culture conditions application system disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information , we have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!


, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture. , can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!