CCC-HSF-1; human skin fibroblasts

Human skin fibroblasts

[Product number]

[Chinese abbreviation]
Cells

[Chinese name]
Human skin fibroblasts

[Background information]
are obtained from periembryonic tissue induced during labor.

[Product Source]
The main source of cells is B and a few well-known universities at home and abroad.

The company provides adherent, semi-adherent suspension, semi-suspension, adherent and suspension Mixing and other cell characteristics, the general B amount of cell culture is, if the cell status is bad, the B amount can be increased to Cell contamination, when the cell confluence is reached, we start sending, so as to maintain good cell viability when the cells are received. Precautions for resuscitating cells
Change the medium on the same day after receiving the cells. Human skin fibroblasts
are all replaced with the customer's own fresh medium, placed in the incubator, and digested the next day.
Observe while digesting. According to the shape of the cells, stop the digestion time and pipe the edges of the cells at half-minute intervals. If the cells can be piped down, the digestion will be terminated. Customers explore for better digestion time.
There is a cell operation manual accompanying the cells, please read it carefully.
Remember to add double-antibodies to reduce the probability of contamination. In addition, freeze the seeds as soon as possible for later use.
The after-sales time is one week, which is limited to quality problems of the cells themselves. Cell problems caused by Party B’s own improper operation, excessive digestion, contamination caused by not adding double-antibodies, etc. are not included in the after-sales scope.
After receiving the cells, if the cells are in poor condition or encounter problems during culture, please contact us as soon as possible on the same day for processing. Timely feedback on the cell status and cell photos on the same day is an important reference for after-sales tracking and processing, so please pay attention to it.
When the cells are first received, fetal bovine serum can be cultured for two or three days to help the cells recover as quickly as possible. After the cells are stabilized, they can be adjusted back.
Among them, the strips are for adherent cells. For details about suspended cells, please see the cell operating instructions

[Product packaging]
One recovery culture bottle or two cryopreservation tubes

[Product Specifications]
*

[Security Level]

[Product Type]
People

[Tissue source]
Skin

[Cell morphology]
Fibroblast-like

[Cell characteristics]
Adherence

Selection and use of cell culture medium
It has simple ingredients and is the first choice for adherent cells.
Divided into high sugar type and low sugar type.
Suitable for cells with low cell density and difficult growth. Such as screening of hybrid cells and screening and culturing of transformed cells after transfection.
One of the most widely used culture media. The first choice for suspension cell culture, mainly for lymphocytes, but also suitable for other cells.
The culture medium for human skin fibroblasts
has complete ingredients and good culture effect.
The culture medium is suitable for mouse and human diploid cell culture.
The culture medium is suitable for single cell isolation and culture and serum-free culture.
The culture medium is particularly suitable for the growth of primary cells and cells that are difficult to culture.
bbr B

[Cell detection]
The cells do not contain Mycoplasma B bacteria, yeast and fungi

[Cultivation conditions]
See cells for details Instructions

[Passaging method]
It is recommended to change the medium every two days

[Freezing conditions]
See the cell instructions for details

[Main literature]
Please refer to relevant published articles for details
Frequently asked questions and solutions
If the culture bottle is broken and the culture medium is leaking, the cells may be contaminated, so we will promptly Make arrangements to help the teacher solve the problem.
The cell floating culture bottle is unopened, wipe the mouth of the bottle with alcohol and place it flat in the incubator. Observe the next day. If most of the cells stick to the bottom of the bottle again, it indicates that the cell viability is normal. The remaining floating cells can be removed by centrifugation and the culture medium can be left for observation. The cells will grow to confluence and be digested and passaged. If the cells still do not adhere to the wall, they will be The cells are collected by centrifugation and transferred to a new culture bottle. Add part of the culture medium to the original culture bottle to continue culturing. Pay attention during observation. Our technical staff will follow up and provide guidance until the problem is solved.
For adherent cells that grow slowly, appropriate methods of increasing the serum concentration in the culture medium or changing the medium every other day can be used to ensure the status and growth rate of the cells.
For adherent cells that grow unevenly, if the cell distribution is obviously uneven during the culture process, that is, the cells in a certain area have overlapped and grown, and there is a blank next to it. At this time, the cells can be digested and re-printed. Spread, adhere to the wall, and add new culture medium for culture.
Precautions for resuscitating cells
Change the medium on the same day after receiving the cells. Replace all cells with the customer's own fresh medium, put them in the incubator, and digest them the next day.
Observe while digesting. According to the shape of the cells, stop the digestion time and pipe the edges of the cells at half-minute intervals. If the cells can be piped down, the digestion will be terminated. Customers explore for better digestion time.
There is a cell operation manual accompanying the cells, please read it carefully.
Remember to add double-antibodies to reduce the probability of contamination. In addition, freeze the seeds as soon as possible for later use.
The after-sales time is one week. Human skin fibroblasts
are limited to the quality problems of the cells themselves, and the cells may be damaged due to Party B’s own improper and non-standard operation, over-digestion, and contamination caused by not adding dual antibodies. The problem is not in the after-sales category.
After receiving the cells, if the cells are in poor condition or encounter problems during culture, please contact us as soon as possible on the same day for processing. Timely feedback on the cell status and cell photos on the same day is an important reference for after-sales tracking and processing, so please pay attention to it.
When the cells are first received, fetal bovine serum can be cultured for two or three days to help the cells recover as quickly as possible. After the cells are stabilized, they can be adjusted back.
Among them, the strips are for adherent cells, and for suspension cells, please see the cell operating instructions
Cells
The company provides background information on organism growth characteristics, source, organ type, morphology, culture conditions, application system, disease tissue freezing Storage conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, human skin fibroblasts
The company provides background information Organism growth characteristics Source organ type Morphology Culture conditions Application system Disease tissue cryopreservation conditions, etc. Recovery and cryopreservation instructions Information, we have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

/Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease, tissue cryopreservation conditions, etc., recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information human skin fibroblasts
organism growth characteristics source organ type morphology culture conditions application system disease tissue cryopreservation conditions, etc. recovery and cryopreservation instructions Information, we have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cells
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell line culture Experience, can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!