Mouse malondialdehyde (MDA) ELISA kit

[Product Name]
Mouse Malondialdehyde Kit
' [Product Usage] Qualitative and Quantitative Analysis of Tested Samples
' [Product Specifications]/(Two Specifications)
' [Detection method] Enzyme immunoassay/enzyme-linked immunoassay
' [Storage conditions] ℃
' [Delivery period] days (available from stock)
' [Product characteristics] Liquid boxed< br/> ' [Product price] Negotiable by phone (including tax and freight, we provide experimental technical guidance and free testing with kits throughout the process)
' [Test kit species] Deer, sheep, chicken, duck, wolf , fish, horses, cattle, humans, rats, dogs, mice, guinea pigs, hamsters, nude mice, potatoes, rabbits, pigs, monkeys and other animals and plants.
' [Sales advantage] Price advantage in the industry, reliable quality, guarantee of replacement if objective quality problems occur
' [Samples to be tested] Body fluids, serum, plasma, cell culture supernatant, urine, tissue Homogenate, atrial water samples, etc.
' Sample collection Before collecting samples, it must be clear whether the components to be detected are stable enough. Specimens that are tested on the same day after collection should be stored at ℃ for later use. If there are special reasons that require periodic collection of specimens, the specimens should be aliquoted in time and stored at ℃ or ℃. Avoid repeated freezing and thawing. Specimens can be stored for hours at ℃ and months at ℃. ℃ can be stored for months. Some hormone specimens require the addition of aprotinin.
' [
Mouse malondialdehyde kit
Shelf life] half a year (six months)
' 1. Experimental principle
' Combined on the surface of the solid carrier The antigen or antibody reacts with the specimen being tested (antigen or antibody). Use washing to remove unbound antigen or antibody. Then add enzyme-labeled antigen or antibody, which is also bound to the solid-phase carrier through reaction. At this time, the amount of enzyme on the solid phase is proportional to the amount of the test substance in the specimen. After adding the substrate for the enzymatic reaction, the substrate is catalyzed by the enzyme into a colored product. The amount of the product is directly related to the amount of the test substance in the specimen. Qualitative or quantitative analysis can be performed based on the depth of the color. According to the actual application method, it can be divided into the following types
'Double-antibody sandwich coats the capture antibody on a solid-phase carrier, adds the antigen to be detected after blocking, and after incubation, adds the enzyme-labeled detection antibody, capture antibody and detection The antibodies can be two monoclonal antibodies targeting different epitopes, or one monoclonal antibody and one polyclonal antibody targeting the same antigen. However, the detection antibodies require enzyme labeling.
' Competition uses the antigen or antibody to be tested to interfere with the pre-designed system. The final color development result is negatively correlated with the degree of interference of the antigen or antibody to be tested. It is mainly used in the detection of proteins with only one epitope.
'Multi-factor detection technology Multiplex chip technology implants ~ capture antibodies in the form of an array in each well of the well plate, and then operates in the same manner, through chemiluminescence (enzyme) and fluorescent fuel (infrared). detection.
'Cytokines produced by stimulated cells are transient, and the kinetics of gene expression of different cytokines vary. Experiments can only detect the content of immunoreactive cytokine proteins, which does not fully represent the level of cytokine biological activity. For example, the use of anti-cytokine antibodies cannot distinguish whether a cytokine protein is an inactive precursor form or an active mature protein. Furthermore, one could detect partially degraded cytokine proteins that are still immunoreactive (e.g., recognize at least two epitopes), when they may have lost their biological activity.
' 2.
Mouse malondialdehyde kit
Experimental process
' / /
' 3. Operation points
'. Choose the appropriate solid Phase carrier
' .Select the appropriate coating buffer
' .Determine the optimal coating amount
' .Add the sample accurately to avoid bubbles.
' 4. Kits
Advantages
' Our company has more than one kit covering species, etc., and the best-selling varieties have the following advantages
' Extremely high-quality and high-quality antibodies and reagents are The basis of the kit
' Accurately and specifically detects target molecules, and the results are accurate and repeatable
' Highly sensitive and can detect /level targets
' Complete range covering humans, rats, and mice , cotton rats, macaques, pigs, horses, dogs and cats, etc.
' Optimized design of detachable well plates, optimized for the detection of serum, plasma, urine samples and cell culture samples
' 5.
Mouse Malondialdehyde Kit
Frequently Asked Questions
' The editor simply compiled a question, the specific content is as follows
', One kit detects several samples ?
'The editor has seen the three answers most often in major forums
') You can make a sample and add a standard curve. You can make a sample and add a standard curve
') Use the test kit To calculate, a quantitative kit can generally make about 100 samples, and a qualitative kit can make about 100 samples. samples.
' How many serum samples can the kit be able to detect? Can the board be reused?
' Generally, if multiple holes are made, one hole is needed to make the standard curve, and the remaining holes can be used to make a sample. This board cannot be reused. And the supporting reagents are only enough for one board.
' I would like to ask a question. I want to use the method to test an antigen in the serum. After checking the information, I found that there is no ready-made kit. Now there is an alternative. One is to buy antibodies and coat yourself. (I checked and other major companies do not have monoclonal antibodies available). Which one is better? Will self-coated test kits be more reliable than domestically produced test kits?
'. If you feel that the technology is good and other companies are worried about it, you can buy the antibody yourself and coat it yourself. This is a troublesome point. You can use it yourself and feel safe and comfortable