B16-F1; mouse melanoma cells

Mouse melanoma cells

Cell number

Cell abbreviation
Cell

Cell name
Mouse melanoma cells< br/>
Detailed Background
is a subline derived from the skin of melanoma B/tumor-bearing mice that produces melanin.

Cell source
The main source of cells is B and a few well-known domestic and foreign universities established departments

Precautions when purchasing cells
After receiving the cells, first observe the cell bottle Whether it is intact and whether the culture medium is leaking or turbid. If the above phenomena occur, please contact us in time.
Read the cell instructions carefully, mouse melanoma cells
Understand the cell-related information, such as cell morphology, medium, serum ratio, required cytokines, etc.
Wipe the surface of the cell bottle with alcohol and observe the cell status under a microscope. Due to transportation problems, a small amount of adherent cells will fall off the bottle wall. Place the cells in an incubator for overnight culture, and then take them out for observation the next day. At this time, most cells will adhere to the wall. If the cells still cannot adhere, please use trypan blue staining to determine the cell viability. If the cell viability is confirmed to be normal, please centrifuge the cells and culture them again with fresh culture medium. If the staining result shows that the cells have no cells. Vitality, please take a photo and contact us in time. After the information is confirmed, we will send it to you for free again.
It is recommended that customers take a few photos of the cells the day before receiving them to record the status of the cells to facilitate communication with the company's technical department.

Packaging specifications
One recovery culture bottle or two cryopreservation tubes

Cell specifications
*/*
Safety level

Cell species
Mouse

Tissue source
Skin

Cell morphology
Epithelial cell-like< br/>
Cell characteristics
Adhesion

Cell fusion
bbr B

Cell detection
Cells do not contain B Mycoplasma, bacteria, yeast and fungi

Three Don’ts of Cell Culture
Raising scientific research cells is a basic skill in biomedical research. I have three little experiences to share with you
Don’t flask the mouth. Most people prefer to close the bottle over the flame of an alcohol burner, feeling that this avoids contamination. I wonder if you are confused about why the culture medium becomes more purple the more you use it? Do you know why? The mouth of the flask is burned. The culture medium generally uses carbonic acid/bicarbonate as a buffer system. When the mouth of the bottle is over the flame, hot air enters the bottle. After tightening the stopper and placing it in the refrigerator, the air becomes cold and the pressure drops suddenly, and the carbonic acid in the culture medium is converted into carbon dioxide and released. If there is less carbonic acid in the culture medium, it will of course become alkaline. If there is no flask mouth, you will find that the rate at which the culture solution becomes alkaline will be greatly slowed down. Of course, it will still become more or less alkaline with more use, because the room temperature is still higher than the temperature in the refrigerator
In fact, preventing contamination at the mouth of the flask is purely a psychological comfort. You might as well try shaking your fingers over the fire a few times, and you will find that there is no burning pain at all. If there are bacteria, shaking them twice won't kill them much. If you want to burn all the bacteria, you have to burn the bottle mouth and stopper red. I have never used flasks in the country. After coming to the United States, I found that this place is more thorough. There is no alcohol lamp at all in the ultra-clean bench.
Don’t look at the cells too often. For beginners, raising cells is like raising a child, so you should check it out when you have time. The worse the cells are maintained, the more often they need to be seen. In fact, looking at the cells does not bring any benefit to the growth of the cells. Mouse melanoma cells, especially primary cells or cells after immunomagnetic bead sorting, have a particularly low density. The growth factors in the culture medium are very limited. The cells finally secrete some growth-promoting factors around them, forming a local environment conducive to growth. You ran to look at the cells, and the culture bottle shook like this, shaking all the factors away. It is often seen that novices are looking at cells all day long, but the cells never grow well. The old hand throws away the cells for several days and the cells grow like crazy.
Don’t be afraid of digestion. During passaging, beginners are often afraid of excessive cell digestion and will terminate digestion early. Before the cells came down, I blew the lamp so hard that the bottle was filled with bubbles. In my opinion, vigorous pipetting causes more damage to the cells than digestion. When my senior brother was doing primary vascular smooth muscle, he digested it overnight and the cells were fine. I usually wait until the cells become completely round before stopping digestion. The cells can be removed with a gentle shake. Also, be gentle and try not to create bubbles. The mechanical stress when the bubble bursts is quite large, and the damage to cells is also great.
See the cell instructions for details

Passaging methods
It is recommended to change the medium every two days

Freezing conditions
See the cell instructions for details

Main literature
Please refer to relevant published articles for details
Operation procedures and precautions after receiving cells
If the cells are adherent cells and are suspended or partially suspended when received status, please centrifuge the suspended cells immediately, add serum-based complete medium to a new culture dish/bottle and continue culturing for another day. At the same time, replace the remaining adherent cells in the original culture bottle with serum-based complete medium for culture for another day. After 3 days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem
Adherent cells grow slowly and the serum concentration should be increased appropriately and cannot exceed the maximum, or it can be based on The growth density of the cells should be considered after trypsin digestion and then transferred to a new culture flask to continue culturing
If the adherent cells grow unevenly and grow into islands, the cells can be digested, resuspended and dispersed. Cells, add fresh culture medium for culture
Please strictly follow aseptic operations for routine cell culture and passage procedures
Aspirate the culture medium in the original culture bottle, rinse the cells twice with B buffer, and add trypsin for digestion Pay attention to the digestion time of cells, usually control the digestion situation under the microscope. When the cell edge shrinks and the adhesion is loose, it is not recommended to digest until the cells float. Remove the trypsin, add complete culture medium, gently pipet the cell layer, and try to remove the trypsin. Blow off the cell layer and disperse it
Transfer part of the cell suspension to a new culture dish/bottle, add appropriate complete culture medium, mix the cell suspension evenly, and culture it in an incubator
Mouse melanoma cells
Pay attention to the changes in the culture medium value, change the medium regularly every week, and repeat the operation or freeze it after the cell density reaches the level
Pay special attention to if you are using a public laboratory or are new to cell culture , it is recommended to add double antibody culture
After receiving the cells, please replace it with fresh culture medium containing serum as soon as possible. If you need to continue to use the original bottle due to special circumstances, please add additional serum and culture medium to the original bottle. The maximum continued use time should not exceed hours
Do not digest the adherent cells immediately on the day they are received. Please change the medium of the cells and place them in an incubator to incubate until the next day before digestion and passaging. Please give priority to the diameter of the culture dish. Subculture
If the culture bottle wall breaks or leaks when signing for receipt, please take photo records in time and contact the laboratory

Main source of cells
B and a few domestic and foreign Established in a famous university, the company provides background information on organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture services. Technical support makes your experiments hassle-free!

Cells
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information on organism growth characteristics, source, organ type, morphology, culture conditions, application department, disease tissue cryopreservation conditions, etc. recovery and Cryopreservation instructions information, we have rich experience in cell line culture and can provide all-round technical support for cell culture, so that your experiments will be hassle-free!

B cells
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information. Growth characteristics of the organism, source, organ type, morphology, culture conditions, application department, disease tissue cryopreservation conditions, etc. recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!

Cells
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information on organism growth characteristics, source, organ type, morphology, culture conditions, application department, disease tissue cryopreservation conditions, etc. recovery and Cryopreservation instructions information, we have rich experience in cell line culture and can provide all-round technical support for cell culture, so that your experiments will be hassle-free!

Cells
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information on organism growth characteristics, source, organ type, morphology, culture conditions, application department, disease tissue cryopreservation conditions, etc. recovery and Cryopreservation instructions information, we have rich experience in cell line culture and can provide all-round technical support for cell culture, so that your experiments will be hassle-free!

Cells
The main source of cells is B and a few well-known universities at home and abroad. Mouse melanoma cells
The company provides background information. Organism growth characteristics. Source. Organ type. Morphology. Culture conditions. Application. It includes information on recovery and cryopreservation instructions such as cryopreservation conditions of diseased tissues. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!

B cells
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information. Growth characteristics of the organism, source, organ type, morphology, culture conditions, application department, disease tissue cryopreservation conditions, etc. recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!

Cells
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information on organism growth characteristics, source, organ type, morphology, culture conditions, application department, disease tissue cryopreservation conditions, etc. recovery and Cryopreservation instructions information, we have rich experience in cell line culture and can provide all-round technical support for cell culture, so that your experiments will be hassle-free!

Cells
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information on organism growth characteristics, source, organ type, morphology, culture conditions, application department, disease tissue cryopreservation conditions, etc. recovery and Cryopreservation instructions information, we have rich experience in cell line culture and can provide all-round technical support for cell culture, so that your experiments will be hassle-free!

Cells
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information on organism growth characteristics, source, organ type, morphology, culture conditions, application department, disease tissue cryopreservation conditions, etc. recovery and Cryopreservation instructions information, we have rich experience in cell line culture and can provide all-round technical support for cell culture, so that your experiments will be hassle-free!

Cells
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information on organism growth characteristics, source, organ type, morphology, culture conditions, application department, disease tissue cryopreservation conditions, etc. recovery and Cryopreservation instructions information, we have rich experience in cell line culture and can provide all-round technical support for cell culture, so that your experiments will be hassle-free!

Cells
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information on organism growth characteristics, source, organ type, morphology, culture conditions, application department, disease tissue cryopreservation conditions, etc. recovery and Cryopreservation instructions information, we have rich experience in cell line culture and can provide all-round technical support for cell culture, so that your experiments will be hassle-free!

Cells
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information on organism growth characteristics, source, organ type, morphology, culture conditions, application department, disease tissue cryopreservation conditions, etc. recovery and Cryopreservation instructions information, we have rich experience in cell line culture and can provide all-round technical support for cell culture, so that your experiments will be hassle-free!

Cells
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information on organism growth characteristics, source, organ type, morphology, culture conditions, application department, disease tissue cryopreservation conditions, etc. recovery and Cryopreservation instructions information, we have rich experience in cell line culture and can provide all-round technical support for cell culture, so that your experiments will be hassle-free!

Cells
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information on organism growth characteristics, source, organ type, morphology, culture conditions, application department, disease tissue cryopreservation conditions, etc. recovery and Cryopreservation instructions information, we have rich experience in cell line culture and can provide all-round technical support for cell culture, so that your experiments will be hassle-free!

Cells
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information on organism growth characteristics, source, organ type, morphology, culture conditions, application department, disease tissue cryopreservation conditions, etc. recovery and Cryopreservation instructions information, we have rich experience in cell line culture and can provide all-round technical support for cell culture, so that your experiments will be hassle-free!

Cells
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information on organism growth characteristics, source, organ type, morphology, culture conditions, application department, disease tissue cryopreservation conditions, etc. recovery and Cryopreservation instructions information, we have rich experience in cell line culture and can provide all-round technical support for cell culture, so that your experiments will be hassle-free!

Cells
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information on organism growth characteristics, source, organ type, morphology, culture conditions, application department, disease tissue cryopreservation conditions, etc. recovery and Cryopreservation instructions information, we have rich experience in cell line culture and can provide all-round technical support for cell culture, so that your experiments will be hassle-free!

Cells
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information on organism growth characteristics, source, organ type, morphology, culture conditions, application department, disease tissue cryopreservation conditions, etc. recovery and Cryopreservation instructions information, we have rich experience in cell line culture and can provide all-round technical support for cell culture, so that your experiments will be hassle-free!

Cells
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information. Mouse melanoma cells
Organ type growth characteristics source organ type morphology culture conditions application It includes information on recovery and cryopreservation instructions such as cryopreservation conditions of diseased tissues. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!

Cells
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information on organism growth characteristics, source, organ type, morphology, culture conditions, application department, disease tissue cryopreservation conditions, etc. recovery and Cryopreservation instructions information, we have rich experience in cell line culture and can provide all-round technical support for cell culture, so that your experiments will be hassle-free!

Cells
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information on organism growth characteristics, source, organ type, morphology, culture conditions, application department, disease tissue cryopreservation conditions, etc. recovery and Cryopreservation instructions information, we have rich experience in cell line culture and can provide all-round technical support for cell culture, so that your experiments will be hassle-free!

Cells
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information on organism growth characteristics, source, organ type, morphology, culture conditions, application department, disease tissue cryopreservation conditions, etc. recovery and Cryopreservation instructions information, we have rich experience in cell line culture and can provide all-round technical support for cell culture, so that your experiments will be hassle-free!