EB-3; human lymphoid Burkitt lymphoma cells

Human lymphoid Br lymphoma cells

Cell number

Cell abbreviation
Cell

Cell name
Human lymphoid Br Lymphoma cells

Detailed background
The cells were derived from B lymphocytes of a 1-year-old black boy with Br lymphoma and were B positive.

Cell source
The main source of cells is B and a few well-known domestic and foreign universities established departments

Precautions when purchasing cells
After receiving the cells, first observe the cell bottle Whether it is intact and whether the culture medium is leaking or turbid. If the above phenomena occur, please contact us in time.
Read the cell instructions carefully, human lymphoid Br lymphoma cells
Understand the cell-related information, such as cell morphology, medium, serum ratio, required cytokines, etc.
Wipe the surface of the cell bottle with alcohol and observe the cell status under a microscope. Due to transportation problems, a small amount of adherent cells will fall off the bottle wall. Place the cells in an incubator for overnight culture, and then take them out for observation the next day. At this time, most cells will adhere to the wall. If the cells still cannot adhere, please use trypan blue staining to determine the cell viability. If the cell viability is confirmed to be normal, please centrifuge the cells and culture them again with fresh culture medium. If the staining result shows that the cells have no cells. Vitality, please take a photo and contact us in time. After the information is confirmed, we will send it to you for free again.
It is recommended that customers take a few photos of the cells the day before receiving them to record the status of the cells to facilitate communication with the company's technical department.

Packaging specifications
One recovery culture bottle or two cryopreservation tubes

Cell specifications
*/*
Safety level

Cell species
Human

Tissue origin
Lymphocytes

Cell morphology
Lymphoblastoid

Cell characteristics
Suspension

Cell fusion
bbr B

Cell detection
Cells do not contain B Mycoplasma, bacteria, yeast and fungi

Three Don’ts of Cell Culture
Raising scientific research cells is a basic skill in biomedical research. I have three little experiences to share with you
Don’t flask the mouth. Most people prefer to close the bottle over the flame of an alcohol burner, feeling that this avoids contamination. I wonder if you are confused about why the culture medium becomes more purple the more you use it? Do you know why? The mouth of the flask is burned. The culture medium generally uses carbonic acid/bicarbonate as a buffer system. When the mouth of the bottle is over the flame, hot air enters the bottle. After tightening the stopper and placing it in the refrigerator, the air becomes cold and the pressure drops suddenly, and the carbonic acid in the culture medium is converted into carbon dioxide and released. If there is less carbonic acid in the culture medium, it will of course become alkaline. If there is no flask mouth, you will find that the rate at which the culture solution becomes alkaline will be greatly slowed down. Of course, it will still become more or less alkaline with more use, because the room temperature is still higher than the temperature in the refrigerator
In fact, preventing contamination at the mouth of the flask is purely a psychological comfort. You might as well try shaking your fingers over the fire a few times, and you will find that there is no burning pain at all. If there are bacteria, shaking them twice won't kill them much. If you want to burn all the bacteria, you have to burn the bottle mouth and stopper red. I have never used flasks in the country. After coming to the United States, I found that this place is more thorough. There is no alcohol lamp at all in the ultra-clean bench.
Don’t look at the cells too often. For beginners, raising cells is like raising a child, so you should check it out when you have time. The worse the cells are maintained, the more often they need to be seen. In fact, looking at the cells does not bring any benefit to the growth of the cells, especially for primary cells or cells with particularly low density after immunomagnetic bead sorting. The growth factors in the culture medium are very limited. The cells finally secrete some growth-promoting factors around them, forming a local environment conducive to growth. You ran to look at the cells, and the culture bottle shook like this, shaking all the factors away. It is often seen that novices are looking at cells all day long, but the cells never grow well. The old hand throws away the cells for several days and the cells grow like crazy.
Don’t be afraid of digestion. During passaging, beginners are often afraid of excessive cell digestion and will terminate digestion early. Before the cells came down, I blew the lamp so hard that the bottle was filled with bubbles. In my opinion, vigorous pipetting causes more damage to the cells than digestion. When my senior brother was doing primary vascular smooth muscle, he digested it overnight and the cells were fine. I usually wait until the cells become completely round before stopping digestion. The cells can be removed with a gentle shake. Also, be gentle and try not to create bubbles. The mechanical stress when the bubble bursts is quite large, and the damage to cells is also great.
See the cell instructions for details

Passaging methods
It is recommended to change the medium every two days

Freezing conditions
See the cell instructions for details

Main literature
Please refer to relevant published articles for details
Operation procedures and precautions after receiving cells
If the cells are adherent cells and are suspended or partially suspended when received status, please centrifuge the suspended cells immediately, add serum-based complete medium to a new culture dish/bottle and continue culturing for another day. At the same time, replace the remaining adherent cells in the original culture bottle with serum-based complete medium for culture for another day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up to solve the problem
Adherent cells grow slowly human lymphoid Br lymphoma cells
Appropriately increase the serum concentration and do not exceed the maximum, or according to the cell growth density, consider trypsin digestion and then transfer to a new culture bottle to continue culturing
Uneven growth If adherent cells are unevenly distributed, they will grow into islands. , the cells can be digested, resuspended and dispersed, and fresh medium added for culture
Please strictly follow the aseptic operation for routine cell culture and passaging procedures
Aspirate the culture medium and B buffer from the original culture bottle Rinse the cells twice and add trypsin for digestion. Pay attention to the digestion time, usually control it under a microscope. Observe the digestion situation under a microscope. When the edges of the cells are shrinking and the adhesion is loose, it is not recommended to digest until the cells are floating. Remove the trypsin and add Complete culture medium, gently pipet the cell layer, try to blow off the cell layer, blow it away
Transfer part of the cell suspension to a new culture dish/bottle, add appropriate complete culture medium, and mix the cell suspension. Evenly, culture in the incubator
Human lymphoid Br lymphoma cells
Pay attention to the changes in culture medium value, change the medium regularly once a week, and repeat the operation or freeze it after the cell density reaches the level
Pay special attention to the fact that if you use a public laboratory or are new to cell culture, it is recommended to add double antibodies for culture
Please replace the cells with fresh culture medium containing serum as soon as possible after receiving the cells. If you need to continue to use the original bottle due to special circumstances, please replace it with the original bottle. For additional serum added to the bottle of culture medium, the original bottle of culture medium should not be used for more than an hour
Do not digest the adherent cells immediately on the day they are received. Please replace the cells with medium and place them in an incubator to incubate until the next day. For subsequent digestion and subculture, please give priority to choosing a petri dish with a larger diameter for subculture
If the wall of the culture bottle is broken or leaks when signing, please take a photo record in time and contact the laboratory

Cell line
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information. Growth characteristics of organisms. Sources. Organ types. Morphology. Culture conditions. Applications. Diseases. Tissue cryopreservation conditions and other recovery and cryopreservation instruction information. We have With rich experience in cell line culture, we can provide comprehensive technical support for cell culture, so that your experiments will be hassle-free!

Cell lines
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information. Growth characteristics of the organism. Source. Organ type. Morphology. Culture conditions. Application system. Disease tissue cryopreservation conditions. Recovery conditions. and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!

Cell lines
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information. Growth characteristics of the organism. Source. Organ type. Morphology. Culture conditions. Application system. Disease tissue cryopreservation conditions. Recovery conditions. and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!

Cell lines
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information. Growth characteristics of the organism. Source. Organ type. Morphology. Culture conditions. Application system. Disease tissue cryopreservation conditions. Recovery conditions. and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!

Cell line
The main source of cells is B and a few well-known universities at home and abroad, human lymphoid Br lymphoma cells
The company provides background information on organism growth characteristics, source organ type and morphology The culture conditions apply, including disease tissue cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!

Cell lines
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information. Growth characteristics of the organism. Source. Organ type. Morphology. Culture conditions. Application system. Disease tissue cryopreservation conditions. Recovery conditions. and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!

Cell line
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information. Growth characteristics of the organism. Source. Organ type. Morphology. Culture conditions. Application department. Disease tissue cryopreservation conditions. Recovery conditions. and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!

Cell lines
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information. Growth characteristics of the organism. Source. Organ type. Morphology. Culture conditions. Application system. Disease tissue cryopreservation conditions. Recovery conditions. and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!

Cell lines
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information. Growth characteristics of the organism. Source. Organ type. Morphology. Culture conditions. Application system. Disease tissue cryopreservation conditions. Recovery conditions. and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!

Cell lines
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information. Growth characteristics of the organism. Source. Organ type. Morphology. Culture conditions. Application system. Disease tissue cryopreservation conditions. Recovery conditions. and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!

Cell line
The main source of cells is B and a few well-known universities at home and abroad, human lymphoid Br lymphoma cells
The company provides background information on organism growth characteristics, source organ type and morphology The culture conditions apply, including disease tissue cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!

Cell lines
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information. Growth characteristics of the organism. Source. Organ type. Morphology. Culture conditions. Application system. Disease tissue cryopreservation conditions. Recovery conditions. and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!

Cell lines
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information. Growth characteristics of the organism. Source. Organ type. Morphology. Culture conditions. Application system. Disease tissue cryopreservation conditions. Recovery conditions. and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!

Cell lines
The main source of cells is B and a few well-known universities at home and abroad. The company provides background information. Growth characteristics of the organism. Source. Organ type. Morphology. Culture conditions. Application system. Disease tissue cryopreservation conditions. Recovery conditions. and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!