Human normal colon epithelial cells CCD 841 CoN

Human normal colon epithelial cells c=r> c=r>
[Cell number] c=r>
C
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[Cell abbreviation] c=r>
C cells
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[Cell name] c=r>
C (human normal colon epithelial cells)
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[Background information] c=r>
See the cell instructions
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[Pay attention to the digestion time when digesting cells. Insufficient digestion will cause damage when pipetting the cells. The general digestion time is Minutes, but based on the fact that most of the cells retract into a spherical shape during microscopic examination, the cells can be pipetted down in a few times. Cell pipetting may damage the cells if the digestion is not sufficient. The formula of cryopreservation solution for cell lines: %B+%. If the adherent cells are unevenly distributed and grow in an island shape, the cells can be digested, resuspended and dispersed, and fresh medium added for culture] c=r> < br/>The main sources of cells are C, Rr, and a few well-known universities at home and abroad
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[Generation] c=r>
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[Specifications] c=r>
Resuscitation culture bottle (one bottle) or cryopreservation tube (two)
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[Number of cells] c=r>
*
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[Biological safety level] c=r>
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[Organism] c=r>
Human< br/> c=r>
[Tissue source] c=r>
Colon
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[Cell morphology] c=r>
Epithelioid
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[Cell characteristics] c=r>
Adhesion
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[Special attention: if using a public laboratory or first contact For cell culture, it is recommended to add double antibodies for culture. Adherent cells should not be digested immediately on the day they are received. Please change the medium and place the cells in an incubator to incubate until the next day before digestion and subculture. Please give priority to a culture dish with a diameter of C for subculture. If the cells are adherent cells and are in a suspended or partially suspended state when received, human normal colon epithelial cells C, please centrifuge the suspended cells immediately, add % serum complete medium to a new culture dish/bottle and continue culturing for another day. At the same time, the remaining adherent cells in the original culture flask were replaced with complete culture medium containing % serum for 1 day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem] c=
% (c)
c=r> < br/>[Cell detection] c=r>
Cells do not contain mycoplasma, bacteria, yeast and fungi
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[Cultivation conditions] c=r>
For details, please refer to the cell instructions
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[Passaging method] c=r>
Recommendation: Change the medium once every two days
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[Crystallization Conditions] c=r>
Please refer to the cell instructions for details
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[Main literature] c=r>
Please refer to relevant published articles for details
Cell reception The final operating procedures and precautions:
If the cells are adherent cells and are in a suspended or partially suspended state when received, please centrifuge the suspended cells immediately and add % serum complete culture medium to a new The culture dish/bottle continues to be cultured for another day while the remaining adherent cells in the original culture bottle are replaced with complete medium containing % serum for another day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem
. Adherent cells grow slowly and increase the serum concentration appropriately (the maximum cannot exceed %) , or according to the cell growth density, trypsin digestion can be considered and then transferred to a new culture bottle to continue culturing.
Uneven growth: If the adherent cells are unevenly distributed and grow into islands, the cells can be Digest, resuspend and disperse the cells, add fresh culture medium for culture
Routine cell culture and passage process (please strictly follow aseptic operation)
Aspirate the culture medium in the original culture bottle, and rinse with B buffer cells twice, add .% trypsin to digest the cells (pay attention to the digestion time, usually control it)
Observe the digestion situation under the microscope. Normal human colon epithelial cells C shrink at the edge of the cells, and when they are loosened (not It is recommended to digest until the cells float), remove the trypsin, add complete culture medium, gently pipet the cell layer, try to blow off the cell layer, blow it loose
, transfer part of the cell suspension to a new culture dish/bottle, Add appropriate complete culture medium, mix the cell suspension evenly, and culture it in the incubator
Pay attention to the changes in the culture medium value, change the medium regularly (weekly), and repeat the operation after the cell density reaches % or Cryopreservation
Special note: (If you use a public laboratory or are new to cell culture, it is recommended to add double antibody culture)
Please replace the cells with fresh culture medium containing % serum as soon as possible after receiving the cells. If you need to continue to use the original bottle under special circumstances, please add additional % serum to the original bottle of culture medium. (The original bottle of culture medium should not be continued to be used for more than an hour.)
Do not digest the adherent cells immediately on the day they are received. Please change the medium of the cells and place them in the incubator to incubate until the next day before digestion and subculture. Please give priority to the culture dish with diameter c for subculture
. If the wall of the culture bottle is broken or leaks when signing for it, please contact us in time. Take photo records and contact the laboratory
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B cells c=r>
. The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines from American cell banks ( More than 3,000 types), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have a wealth of cell With extensive experience in cell culture, we can provide a full range of technical support for cell culture, making your experiments hassle-free!
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[]Cell c=r>
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing Cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide cells Provide comprehensive technical support so that your experiments will be hassle-free!
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B cells c=r>
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cells strain organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide cell culture Comprehensive technical support makes your experiments hassle-free!
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Cells c=r>
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell lines Organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide comprehensive cell culture services. With comprehensive technical support, your experiments will be hassle-free!
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Cells c=r>
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell lines Organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide comprehensive cell culture services. With comprehensive technical support, your experiments will be hassle-free!
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C[C] cells c=r>
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks. , providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can Provide comprehensive technical support for cell culture so that your experiments will be hassle-free!
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Cells c=r>
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell lines Organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide comprehensive cell culture services. With comprehensive technical support, your experiments will be hassle-free!
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R cells c=r>
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cells strain organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide cell culture Comprehensive technical support makes your experiments hassle-free!
Human normal colon epithelial cells=r>
Cell c=r>
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks. , providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can Provide comprehensive technical support for cell culture so that your experiments will be hassle-free!
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B cells c=r>
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cells strain organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide cell culture Comprehensive technical support makes your experiments hassle-free!
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Cells c=r>
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell lines Organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide comprehensive cell culture services. With comprehensive technical support, your experiments will be hassle-free!
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Cells c=r>
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell lines Organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide comprehensive cell culture services. With comprehensive technical support, your experiments will be hassle-free!
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Cells c=r>
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell lines Organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide comprehensive cell culture services. With comprehensive technical support, your experiments will be hassle-free!
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[]Cell c=r>
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing Cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide cells Provide comprehensive technical support so that your experiments will be hassle-free!