hCMEC/D3<Immortalized human brain microvascular endothelial cells>

r/&;Immortalized human brain microvascular endothelial cells&;
r
[Cell number]
[Cell abbreviation] /cell
[Cell name] /( Immortalized human brain microvascular endothelial cells)
[Background information] See the cell instructions
[Cell sources] The main sources of cells are,,,,r,,,RB,B,, and a few well-known universities at home and abroad. System
[Generation]
[Specification] Recovery culture bottle (one bottle) or cryopreservation tube (two)
[Number of cells] * ()
[Biology Safety level]
[Organism] Human
[Tissue source] Brain; tissue: blood-brain barrier
[Cell morphology] Endothelium
[Cell characteristics] Adhesion
[Cell viability] % (bb B)
[Cell detection] Cells do not contain -, B, mycoplasma, bacteria, yeast and fungi
[Cultivation conditions] Please refer to the cell instructions for details
[Passaging Method] Recommendation:-: Change the medium once every two days
[Freezing conditions] Please refer to the cell instructions for details
[Main literature] Please refer to relevant published articles for details
Analysis and recommendations on common experimental problems of thawing cells Solution:
rWhen thawing frozen cells, it was found that problems such as low survival rate, large amounts of cell debris, and slow growth occurred. What is the cause and how can we solve it? The following are some solutions summarized by domestic and foreign cell culture experts for thawing cell experimental problems!
rPossible reasons for low survival rate and recommended solutions are as follows:
r. Cell lysis during thawing process
r/&;Immortalized human brain microvascular endothelial cells&;
r Recommended solution: A certain amount of cell death can be expected, so the concentration of cells should be high enough to take this loss into account. Starting concentration is *() to *() cells/ml.
r. Problems during thawing
r Recommended solution: Store frozen cultures at -°C to -°C for - days, but this is not the preferred method of preservation. Culture should be started immediately after sufficient thawing at ℃.
r. Allergy to freezing liquid
r Recommended solution:. Completely or partially replace the culture medium and reduce the amount of freezing liquid in the culture medium. Replacing the culture fluid after hours will remove all the freezing fluid. B. Allow more time for the culture to recover. Sometimes it takes several weeks for cells to form a monolayer or a dense suspension, depending on the age or passage number of the cells at the time of freezing or their position in the growth phase. The optimal conditions for freezing are in the logarithmic phase.
r. Age of frozen stock cells or age of culture at time of freezing
r Recommended solution: Thaw recently frozen cells. The longer the cells remain frozen, the lower the survival rate. Check when and how to freeze cells. Cells should be in logarithmic phase when frozen.
rPossible causes and recommended solutions for the occurrence of large amounts of cell debris are as follows:
r. The cell density is too low during freezing: Recommended solution: shorten the time during the thawing process.
r/&;Immortalized human brain microvascular endothelial cells&;
After taking out the cells, immediately immerse the cryopreservation tube in a water bath at ℃ and shake until completely melted, and then quickly transfer to in preheated culture medium.
r. Improper freezing process: Recommended solution: Thaw tubes from different freezers. Ensure proper technique is used during cryopreservation and that the appropriate amount and appropriate freezing fluid is used.
rPossible reasons for slow growth and recommended solutions are as follows:
r. Size of culture flask: The general solution is that some cells tend to maintain a certain density in the culture medium. Transfer the culture to a smaller flask to increase cell density; e.g., transfer from flask to flask or flasks, depending on the volume of culture medium.
r. Cell density is too low: The usual solution is to increase the freezing density of cells of future frozen species, or use smaller culture vessels, or thaw multiple test tubes for culture. --, the company provides background information, organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
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r/&;Immortalized human brain microvascular endothelial cells&;
r R[RR], the company provides background information, organisms, growth characteristics, sources, organs, types, morphology, culture conditions, and applications , lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!
R, the company provides background information, organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have a wealth of information With rich experience in cell line culture, we can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
, the company provides background information, organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have a wealth of With experience in cell line culture, we can provide a full range of technical support for cell culture, making your experiments hassle-free!
- The company provides background information, organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have a wealth of information With rich experience in cell line culture, we can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
- The company provides background information, organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have a wealth of information With rich experience in cell line culture, we can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
- The company provides background information, organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have a wealth of information With rich experience in cell line culture, we can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
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