Human peritoneal mesothelial cells HMrSV5

Human peritoneal mesothelial cells c=r> c=r>
[Cell number] c=r>
C
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[Cell abbreviation] c=r>
r cells
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[Cell name] c=r>
r (human peritoneal mesothelial cells)
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[Background information] c=r>
See the cell instructions
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[Pay attention to the digestion time when digesting cells. Insufficient digestion will cause damage when pipetting the cells. The general digestion time is Minutes, but based on the fact that most of the cells retract into a spherical shape during microscopic examination, the cells can be pipetted down in a few times. Cell pipetting may damage the cells if the digestion is not sufficient. The formula of cryopreservation solution for cell lines: %B+%. If the adherent cells are unevenly distributed and grow in an island shape, human peritoneal mesothelial cells can be digested, resuspended and dispersed, and then added with fresh medium for culture. 】 c=
The main sources of cells are C, Rr, and a few well-known universities at home and abroad
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【Generation】 c=r>
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[Specification] c=r>
Resuscitation culture bottle (one bottle) or cryopreservation tube (two)
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[Number of cells] c= r>
*
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【Biosafety Level】 c=r>
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【Organism】 c=r>
Human
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[Tissue source] c=r>
Mesothelium
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[Cell morphology] c=r> < br/>Epithelioid
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[Cell Characteristics] c=r>
Adhesion
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[Special Note: If using public In the laboratory or for the first time contacting cell culture, it is recommended to add double antibody culture. Adherent cells should not be digested immediately on the day they are received. Please change the medium of the cells and place them in an incubator to incubate until the next day before digestion and passaging. Please give priority to culture with diameter c. If the cells are adherent cells and are suspended or partially suspended when received, please centrifuge the suspended cells immediately, add % serum complete culture medium to a new culture dish/bottle and continue culturing for another day. The remaining adherent cells in the original culture flask were replaced with complete culture medium containing % serum for 1 day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem] c=r>
% (c)
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[Cell detection] c=r>
Cells do not contain mycoplasma, bacteria, yeast and fungi
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[Cultivation conditions] c=r>
See cell instructions for details
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[Passaging method] c=r>
Recommendation: Change the medium once every two days
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[ Freezing conditions] c=r>
Please refer to the cell instructions for details
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[Main literature] c=r>
Please refer to relevant published articles for details
Operation procedures and precautions after receiving cells:
If the cells are adherent cells and are suspended or partially suspended when received, please centrifuge the suspended cells immediately and add % serum complete culture medium to The new culture dish/bottle continues to be cultured for another day while the remaining adherent cells in the original culture bottle are replaced with % serum complete culture medium for another day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem
. Adherent cells grow slowly and increase the serum concentration appropriately (the maximum cannot exceed %) , or according to the cell growth density, trypsin digestion can be considered and then transferred to a new culture bottle to continue culturing.
Uneven growth: If the adherent cells are unevenly distributed and grow into islands, the cells can be Digest, resuspend and disperse the cells, add fresh culture medium for culture
Routine cell culture and passage process (please strictly follow aseptic operation)
Aspirate the culture medium in the original culture bottle, and rinse with B buffer cells twice, add .% trypsin to digest the cells (pay attention to the digestion time, usually control it)
Observe the digestion situation under the microscope. Human peritoneal mesothelial cells shrink at the edge of the cells, and when they are loosened (not It is recommended to digest until the cells float), remove the trypsin, add complete culture medium, gently pipet the cell layer, try to blow off the cell layer, blow it loose
, transfer part of the cell suspension to a new culture dish/bottle, Add appropriate complete culture medium, mix the cell suspension evenly, and culture it in the incubator
Pay attention to the changes in the culture medium value, change the medium regularly (weekly), and repeat the operation after the cell density reaches % or Cryopreservation
Special note: (If you use a public laboratory or are new to cell culture, it is recommended to add double antibody culture)
Please replace the cells with fresh culture medium containing % serum as soon as possible after receiving the cells. If you need to continue to use the original bottle under special circumstances, please add additional % serum to the original bottle of culture medium. (The original bottle of culture medium should not be continued to be used for more than an hour.)
Do not digest the adherent cells immediately on the day they are received. Please change the medium of the cells and place them in the incubator to incubate until the next day before digestion and subculture. Please give priority to the culture dish with diameter c for subculture
. If the wall of the culture bottle is broken or leaks when signing for it, please contact us in time. Take photo records and contact the laboratory
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Human peritoneal mesothelial cells rc=r>
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