IPEC-J2 porcine small intestinal epithelial cells

Porcine small intestinal epithelial cells
(Pig small intestinal epithelial cells)

[Cell number]

[Cell abbreviation]
Cells

[Cell name]
(Pig small intestinal epithelial cells)

[Background information]
See cell instructions

[Cell source]
The main source of cells, pig small intestinal epithelial cells
, and a few famous domestic and foreign universities established departments

[Generations]

[Specifications]
Resuscitation culture Bottles (one bottle) or cryopreservation tubes (two)

[Number of cells]
*

[Biosafety level]
< br/>[Organism]
Human

[Tissue source]
Small intestine

[Cell morphology]
See the cell instructions section for details

【Cell Characteristics】
Adhesion

【Cell Viability】
)

【Cell Detection】
Cells do not contain mycoplasma, bacteria, yeast and fungi

[Culture conditions]
See cell instructions for details

[Passaging method]
Two recommended Change the medium once a day

[Freezing conditions]
See the cell instructions for details

[Main literature]
Please check the relevant published articles for details
Operation procedures and precautions after receipt
If the cells are adherent cells and are in a suspended or partially suspended state when received, please centrifuge the suspended cells immediately and add % serum complete culture medium to new cells. The culture dish/bottle continues to be cultured for another day while the remaining adherent cells in the original culture bottle are replaced with complete culture medium containing % serum for another day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, pig small intestinal epithelial cells
Please contact the laboratory technician in time and they will follow up and solve the problem
. Adherent cells grow slowly and increase appropriately. Serum concentration (maximum cannot exceed %), or according to the cell growth density, trypsin digestion can be considered, and then transferred to a new culture bottle to continue culturing
If the adherent cells grow unevenly and are unevenly distributed, they will form islands. To grow like this, the cells can be digested, resuspended and dispersed, and added with fresh culture medium for culture
Routine cell culture passage process (please strictly follow aseptic operation)
Aspirate the culture in the original culture bottle Base, rinse the cells twice with buffer, add .% trypsin to digest the cells (pay attention to the digestion time, usually control it)
Observe the digestion situation under the microscope, when the edge of the cell shrinks and the adhesion becomes loose (do not It is recommended to digest until the cells float), remove the trypsin, add complete culture medium, gently pipet the cell layer, try to blow off the cell layer, blow it loose
, transfer part of the cell suspension to a new culture dish/bottle, Add appropriate complete culture medium, mix the cell suspension evenly, and culture it in the incubator
Pay attention to the changes in the culture medium value, change the medium regularly (weekly), and repeat the operation after the cell density reaches % or Cryopreservation


The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell line organisms, growth characteristics, and sources. , organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, allowing you to Experiments are no longer troublesome

The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the American cell bank, providing cell line organisms, growth characteristics, Source, organ, type, morphology, culture conditions, application, line, disease, tissue, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that Your experiment will no longer be troublesome


The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the American cell bank, providing cell lines, organisms, growth Characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide all-round technical support for cell culture. , allowing you to experiment without any worries


The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell line organisms , growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture services. Technical support allows you to experiment without any worries
Porcine small intestinal epithelial cells


The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (three Thousands of species), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have a wealth of cell lines With culture experience, we can provide a full range of technical support for cell culture, so that your experiments will be hassle-free


The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines from American cell banks. (more than 3,000 types), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have a wealth of information With experience in cell line culture, we can provide a full range of technical support for cell culture, so that your experiments will be trouble-free

The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (three Thousands of species), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have a wealth of cell lines With culture experience, we can provide a full range of technical support for cell culture, so that your experiments will be hassle-free


The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines from American cell banks. (more than 3,000 types), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have a wealth of information With experience in cell line culture, we can provide a full range of technical support for cell culture, so that your experiments will be trouble-free

The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (three Thousands of species), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have a wealth of cell lines With culture experience, we can provide a full range of technical support for cell culture, so that your experiments will be hassle-free


The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines from American cell banks. (more than 3,000 types), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have a wealth of information With experience in cell line culture, we can provide a full range of technical support for cell culture, so that your experiments will be trouble-free


The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines from American cell banks. Cell lines (more than 3,000 types), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information, we have With rich experience in cell line culture, we can provide a full range of technical support for cell culture, so that your experiments will be hassle-free

The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines from American cell banks. (more than 3,000 types), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other information on recovery and cryopreservation instructions. We have a wealth of With experience in cell line culture, we can provide a full range of technical support for cell culture, so that your experiments will be trouble-free

The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (three Thousands of species), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have a wealth of cell lines With culture experience, we can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome

The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than three thousand) from American cell banks. species), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture , can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome

The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks. , providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can Provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome

The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing Cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide cells We provide all-round technical support so that your experiments will be hassle-free


The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks. , providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can Provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome


The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than three thousand) from American cell banks. species), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture , can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome


The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (three Thousands of species), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have a wealth of cell lines With culture experience, we can provide a full range of technical support for cell culture, so that your experiments will be trouble-free


The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines from American cell banks. (more than 3,000 types), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other information on recovery and cryopreservation instructions. We have a wealth of With experience in cell line culture, we can provide a full range of technical support for cell culture, so that your experiments will be hassle-free


The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines from the American cell bank. Cell lines (more than 3,000 types), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information, we have With rich experience in cell line culture, we can provide a full range of technical support for cell culture, so that your experiments will be hassle-free

The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines from American cell banks. (more than 3,000 types), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other information on recovery and cryopreservation instructions. We have a wealth of With experience in cell line culture, we can provide a full range of technical support for cell culture, so that your experiments will be hassle-free


The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines from the American cell bank. Cell lines (more than 3,000 types), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information, we have With rich experience in cell line culture, we can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome


The company specializes in providing normal cell lines and tumor cell lines from American cell banks. , cancer cell lines (more than 3,000 types), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information, We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome


The company specializes in providing normal cell lines and tumor cells derived from American cell banks. Cell lines and cancer cell lines (more than 3,000 types), providing recovery and cryopreservation instructions for cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions, etc. Information, we have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome

The company specializes in providing normal cell lines and tumor cell lines from American cell banks. , cancer cell lines (more than 3,000 types), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information, We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome


The company specializes in providing normal cell lines and tumor cells derived from American cell banks. Cell lines and cancer cell lines (more than 3,000 types), providing recovery and cryopreservation instructions for cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions, etc. Information, we have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
Porcine small intestinal epithelial cells

, the company specializes in providing American cell banks Sources of normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, and cryopreservation conditions Waiting for recovery and cryopreservation instructions, we have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free

The company specializes in providing normal sources of American cell banks. Cell lines, tumor cell lines, cancer cell lines (more than 3,000 types), providing recovery of cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions, etc. and cryopreservation instruction information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free


The company specializes in providing American cell banks Sources of normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, and cryopreservation conditions We have extensive experience in cell line culture and can provide all-round technical support for cell culture so that your experiments will be hassle-free