GIST-T1 gastrointestinal stromal tumor cells

Gastrointestinal stromal tumor cells
(Gastrointestinal stromal tumor cells)

[Cell number]

[Cell abbreviation]
Cell< br/>
[Cell name]
(Gastrointestinal stromal tumor cells)

[Background information]
See cell instructions

【Cell source】
The main source of cells, gastrointestinal stromal tumor cells
, and a few well-known domestic and foreign universities established departments

[Generation]
< br/>[Specification]
Resuscitation culture bottle (one bottle) or cryopreservation tube (two)

[Number of cells]
*

【Biosafety Level】

【Organism】
Human

【Tissue Source】
Gastrointestinal Tract

【Cell morphology】
See the cell instructions section for details

【Cell characteristics】
Adhesion

【Cell viability】
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[Cell detection]
Cells do not contain mycoplasma, bacteria, yeast and fungi

[Cultivation conditions]
See cell instructions for details

[Passaging method]
It is recommended to change the medium every two days

[Freezing conditions]
See the cell instructions for details

[Main literature 】
Please refer to relevant published articles for details
Operation procedures and precautions after receipt
If the cells are adherent cells and are suspended or partially suspended when received, please suspend them. Centrifuge the cells immediately, add % serum complete medium to a new culture dish/bottle and continue culturing for another day. At the same time, the remaining adherent cells in the original culture flask are replaced with % serum complete medium for another day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem
. Adherent cells grow slowly and increase the serum concentration appropriately (the maximum cannot exceed %) , or according to the cell growth density, trypsin digestion can be considered and then transferred to a new culture bottle to continue culturing
If the adherent cells grow unevenly and are unevenly distributed, gastrointestinal stromal tumor cells
To grow in an island shape, the cells can be digested, resuspended and dispersed, and added with fresh medium for culture
The routine cell culture and passage process (please strictly follow the aseptic operation)
Aspirate out the original culture bottle Rinse the cells twice with culture medium and buffer, add .% trypsin to digest the cells (pay attention to the digestion time, usually control it within)
Observe the digestion situation under the microscope, shrink the cells at the edge, and loosen the adhesion (It is not recommended to digest until the cells are floating.) Remove the trypsin, add complete culture medium, gently pipet the cell layer, try to blow off the cell layer, blow it loose
, transfer part of the cell suspension to a new culture dish/ In the bottle, add appropriate complete culture medium, mix the cell suspension evenly, and culture it in the incubator
Pay attention to the changes in the culture medium value, change the medium regularly (weekly), and repeat after the cell density reaches % operation or cryopreservation

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Gastrointestinal stromal tumor cells

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