BV173 human peripheral blood B cells

Human peripheral blood cells
(Human peripheral blood cells)

[Cell number]

[Cell abbreviation]
Cells

[ Cell name]
(Human peripheral blood cells)

[Background information]
See cell instructions

[Cell source]
Main source of cells , human peripheral blood cells
, and a few well-known universities at home and abroad to establish departments

[Generations]

[Specifications]
Resuscitation culture bottle (one bottle ) or cryopreservation vials (two)

[Number of cells]
*

[Biosafety level]

[ Organism]
Human

[Tissue source]
Liver

[Cell morphology]
Cellular precursor leukemia
< br/>【Cell Characteristics】
Suspension

【Cell Viability】
)

【Cell Detection】
Cells do not contain, mycoplasma , bacteria, yeast and fungi

[Culture conditions]
See cell instructions for details

[Passaging method]
It is recommended to change the medium once every two days
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[Freezing Conditions]
Please refer to the cell instructions for details

[Main Documents]
Please refer to relevant published articles for details
Operation procedures after receipt Notes
If the cells are adherent cells and are suspended or partially suspended when received, please centrifuge the suspended cells immediately and add % serum complete culture medium to a new culture dish/bottle to continue. At the same time, the remaining adherent cells in the original culture flask were replaced with complete culture medium containing % serum for the first day of culture. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem
. Adherent cells grow slowly and increase the serum concentration appropriately (the maximum cannot exceed %) , or according to the cell growth density, trypsin digestion can be considered and then transferred to a new culture bottle to continue culturing
If the adherent cells grow unevenly and grow into islands, the cells can be digested , resuspend and disperse the cells, add fresh culture medium for culture
Routine cell culture and passage process (please strictly follow aseptic operation)
Aspirate the culture medium in the original culture bottle, and rinse both cells with buffer. times, add .% trypsin to digest the cells (pay attention to the digestion time, usually control it within)
Observe the digestion situation under the microscope, shrink the edge of the cells, and when human peripheral blood cells
are loosely attached (not recommended Digest until the cells float), remove the trypsin, add complete culture medium, gently pipet the cell layer, try to blow off the cell layer, blow it loose
, transfer part of the cell suspension to a new culture dish/bottle, add Use appropriate complete culture medium, beat the cell suspension evenly, and culture it in the incubator
Pay attention to the changes in the culture medium value, change the medium regularly (weekly), repeat the operation or freeze after the cell density reaches % Cun

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Human peripheral blood cells

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Human peripheral blood cells

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