CATH.a|Neuron Cell

.|Neuron cell b :r
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[Cell number] b :r
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[Cell abbreviation] b :r
.cell
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[Cell name] b :r
.(Neuron cell)
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[Background information] b :r
See the instructions for cells
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[Pay attention to the digestion time when digesting cells. Insufficient digestion will cause damage when pipetting the cells. The general digestion time is minutes, but it is generally based on the fact that most of the cells shrink into a spherical shape during microscopy. The cells can be pipetted down and digested in just one time. Insufficient cell pipetting can easily damage the cells. Cell line cryopreservation solution formula: If B+ adherent cells are unevenly distributed and grow in an island shape, the cells can be digested, resuspended and dispersed, and the cells can be added to fresh medium for culture] b:r
The main source of cells is ZRr and A few well-known universities at home and abroad have established departments
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[Generation] b :r
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[Specifications] b :r
Resuscitation culture bottle ( One bottle) or cryopreserved vials (two)
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[Number of cells] b :r
*
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[Biological safety Level】b :r
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【Organism】b :r
Mouse
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【Tissue source】b :r
Brain
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[Cell morphology] b :r
Neuron cells
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[Cell characteristics] b :r
Post Mix wall and suspension
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[Special note: If you are using a public laboratory or are new to cell culture.|Neuron cells
It is recommended to add double antibodies to culture adherent cells and do not do so on the day they are received. For immediate digestion, please replace the cells with medium and place them in an incubator to incubate until the next day before digestion and subculture. Please give priority to choosing a diameter of culture dish for subculture. If the cells are adherent cells and are in a suspended or partially suspended state when received, please suspend them. Immediately centrifuge the cells and add serum-based complete medium to a new culture dish/bottle to continue culturing for another day. At the same time, the remaining adherent cells in the original culture flask are replaced with serum-based complete medium for culture for another day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem] b
(b)
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[ Cell detection】b:r
The cells do not contain Mycoplasma B bacteria, yeast and fungi
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[Culture conditions]b:r
See the cell instructions for details
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[Passaging method] b :r
Recommendation: Change the medium once every two days
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[Freezing conditions] b :r
See cells for details Instructions
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[Main literature] b :r
Please refer to relevant published articles for details
Operation procedures and precautions after receiving cells
If the cells are adherent When parietal cells are received in a suspended or partially suspended state, please immediately centrifuge the suspended cells and add serum-added complete medium to a new culture dish/bottle to continue culturing. At the same time, replace the remaining adherent cells in the original culture bottle with serum. of complete medium culture days. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem
The adherent cells grow slowly and the serum concentration should be increased appropriately (the maximum cannot be exceeded) or according to the The growth density of the cells should be considered after trypsin digestion and then transferred to a new culture flask to continue culturing
Uneven growth: If the adherent cells appear to be unevenly distributed and grow in an island shape, the cells can be digested, resuspended, and added to fresh culture. Culture medium
Routine cell culture and passaging process (please strictly follow aseptic operation)
Aspirate the medium B buffer in the original culture bottle and rinse the cells twice. Add trypsin to digest the cells (pay attention to the digestion The time is usually controlled at)
Observe the digestion situation under the microscope. | Neuron cells
When the edges of the cells shrink and become loose (it is not recommended to digest until the cells float), remove the trypsin and add complete culture medium and gently pipette the cells. Try to blow off the cell layer as much as possible
Transfer part of the cell suspension to a new culture dish/bottle, add appropriate complete culture medium, mix the cell suspension evenly, and culture it in the incubator
Pay attention to culture Changes in the base value, change the medium regularly (weekly), repeat the operation or freeze storage after the cell density reaches the level
Special note: (If you use a public laboratory or are new to cell culture, it is recommended to add double antibody culture)
Please replace the cells with fresh culture medium containing serum as soon as possible after receiving the cells. If you need to continue to use the original bottle due to special circumstances, please add additional serum to the original bottle of culture medium (the maximum time of continued use of the original bottle of culture medium should not exceed hours)< br/> Do not digest the adherent cells immediately on the day they are received. Please change the medium of the cells and place them in an incubator to incubate until the next day before digesting and subscribing. Please give priority to choosing a culture dish with a diameter for subculturing
If a culture bottle appears when signing for receipt. In case of wall rupture, leakage, etc., please take photo records in time and contact the laboratory
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Cell line b:r
The company specializes in providing normal cell lines, tumor cell lines, and cancer cells from the US cell bank. Cell lines (more than 3,000 types) provide cell lines, organism growth characteristics, source, organ type, morphology, culture conditions, application system, disease tissue cryopreservation conditions, etc., recovery and cryopreservation instruction information. We have rich experience in cell line culture and can provide a full range of cell culture services. Technical support makes your experiments hassle-free!
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Cell line b :r
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank. Provides cell lines, organism growth characteristics, source organs Type, morphology, culture conditions, application, disease tissue cryopreservation conditions, etc. Recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome!
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[]Cell line b :r
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell lines with biological growth characteristics Source organ type, morphology, culture conditions, application system, disease tissue cryopreservation conditions, etc. Recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide all-round technical support for cell culture so that your experiments will be hassle-free!
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Cell lines b :r
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing sources of cell lines and biological growth characteristics. Organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, etc. Recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome!
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[] Cell line b :r
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cell lines for biological growth. Characteristics, source organ type, morphology, culture conditions, application system, disease tissue cryopreservation conditions, etc. Recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome!
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Cell lines b :r
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cell lines, organism growth characteristics, source organs Type, morphology, culture conditions, application, disease tissue cryopreservation conditions, etc. Recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome!
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Cell line b :r
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank. Provides cell lines, organism growth characteristics, source organs Type, morphology, culture conditions, application, disease tissue cryopreservation conditions, etc. Recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome!
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Cell lines b :r
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cell lines with biological growth characteristics Source organ type, morphology, culture conditions, application system, disease tissue cryopreservation conditions, etc. Recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide all-round technical support for cell culture so that your experiments will be hassle-free!
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r cell linesb :r
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the American cell bank, providing cell lines with biological growth characteristics Source organ type, morphology, culture conditions, application system, disease tissue cryopreservation conditions, etc. Recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide all-round technical support for cell culture so that your experiments will be hassle-free!
.|Neuron cells
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[ ] Cell line b :r
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines from the U.S. cell bank (more than three thousand species) provide cell lines, organism growth characteristics, source, organ type, morphology, culture conditions, application system, disease tissue cryopreservation conditions, etc. recovery and cryopreservation instruction information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture to allow you to experiment again. No fuss!
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Cell lines b :r
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing sources of cell lines with biological growth characteristics. Organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, etc. Recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome!
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[] Cell line b :r
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cell lines for biological growth. Characteristics, source organ type, morphology, culture conditions, application system, disease tissue cryopreservation conditions, etc. Recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome!
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R cell line b :r
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cell lines with biological growth characteristics Source organ type, morphology, culture conditions, application system, disease tissue cryopreservation conditions, etc. Recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide all-round technical support for cell culture so that your experiments will be hassle-free!