MH7A|Rheumatoid arthritis synovial fibroblasts

|Rheumatoid arthritis synovial fibroblasts b :r
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[Cell number] b :r
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[Cell abbreviation] b :r < br/>Cells
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[Cell name] b :r
(Rheumatoid arthritis synovial fibroblasts)
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[Background Information】b:r
The synovial cells of patients with chronic indirect leucoma were changed by したもの. bに濜しをproducesするという.
. The cells can be pipetted down in just a few times and digestion is insufficient. Cell pipetting can easily damage the cells. Cell line cryopreservation solution formula: If B+ adherent cells are unevenly distributed and grow in an island shape, the cells can be digested, resuspended and dispersed, and the cells can be added to fresh medium for culture] b:r
The main source of cells is ZRr and A few well-known universities at home and abroad have established departments
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[Generation] b :r
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[Specifications] b :r
Resuscitation culture bottle ( One bottle) or cryopreserved vials (two)
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[Number of cells] b :r
*
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[Biological safety Level】b :r
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【Organism】b :r
Human
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【Organization source】b :r
Synovial membrane
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【Cell morphology】b :r
Fibroblast-like
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【Cell characteristics】b :r
Adhesion
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[Special note: If you use a public laboratory or are new to cell culture | Rheumatoid arthritis synovial fibroblasts
It is recommended to add double antibodies to culture adherent cells to collect Do not digest immediately on the same day. Please change the medium of the cells and place them in an incubator to incubate until the next day before digesting and passaging. Please give priority to choosing a petri dish with a larger diameter for subculture. If the cells are adherent cells, they will be in a suspended or partially suspended state when received. Please centrifuge the suspended cells immediately and add serum-based complete medium to a new culture dish/bottle to continue culturing for another day. At the same time, replace the remaining adherent cells in the original culture bottle with serum-based complete medium for culture for another day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem] b
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[ Cell detection】b:r
The cells do not contain Mycoplasma B bacteria, yeast and fungi
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[Culture conditions]b:r
See the cell instructions for details
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[Passaging method] b :r
Recommendation: Change the medium once every two days
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[Freezing conditions] b :r
See cells for details Instructions
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[Main documents] b :r
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Operation procedures and precautions after receiving cells
If the cells are adherent cells and are received in a suspended or partially suspended state, please transfer the suspended cells Immediately centrifuge the cells and add serum-containing complete medium to a new culture dish/bottle to continue culturing for another day. At the same time, the remaining adherent cells in the original culture flask are replaced with serum-containing complete medium and cultured for another day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem
The adherent cells grow slowly and the serum concentration should be increased appropriately (the maximum cannot be exceeded) or according to the The growth density of the cells should be considered after trypsin digestion and then transferred to a new culture flask to continue culturing
Uneven growth: If the adherent cells appear to be unevenly distributed and grow in an island shape, the cells can be digested, resuspended, and added to fresh culture. Culture medium
Routine cell culture and passaging process (please strictly follow aseptic operation)
Aspirate the medium B buffer in the original culture bottle and rinse the cells twice. Add trypsin to digest the cells (pay attention to the digestion The time is usually controlled)
Observe the digestion situation under the microscope | Rheumatoid arthritis synovial fibroblasts
When the edges of the cells shrink and become loose (it is not recommended to digest until the cells float), remove the trypsin and culture it completely Gently pipe the cell layer to blow off the cell layer as much as possible
Transfer part of the cell suspension to a new culture dish/bottle, add appropriate complete culture medium, mix the cell suspension evenly, and culture it in the incubator< br/> Pay attention to the changes in the culture medium value and change the medium regularly (weekly). Repeat the operation or freeze it after the cell density reaches the level. Special note: (If you use a public laboratory or are new to cell culture, it is recommended to add double antibodies. Culture)
Please replace the cells with fresh culture medium containing serum as soon as possible after receiving the cells. If you need to continue to use the original bottle due to special circumstances, please add additional serum to the original bottle of culture medium (the original bottle of culture medium can continue to be used for the longest time It should not exceed an hour)
Do not digest the adherent cells immediately on the day they are received. Please change the medium of the cells and place them in an incubator to incubate until the next day before digestion and subculture. Please give priority to the diameter of the culture dish for subculture
For example If the culture bottle wall breaks and leaks when signing for receipt, please take photo records in time and contact the laboratory
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Cell lineb:r
The company specializes in providing normal cells from the American cell bank. Tumor cell lines Cancer cell lines (more than 3,000 types) Provide cell lines, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, etc. Recovery and cryopreservation instructions information We have rich experience in cell line culture and can provide Comprehensive technical support for cell culture makes your experiments hassle-free!
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Cell lines b :r
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing sources of cell lines and biological growth characteristics. Organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, etc. Recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome!
Bb :r
Cell lines b :r
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing sources of cell lines and biological growth characteristics. Organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, etc. Recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome!
Bb :r
Cell lines b :r
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing sources of cell lines and biological growth characteristics. Organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, etc. Recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome!
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Cell lines b :r
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cell lines, organism growth characteristics, source organs Type, morphology, culture conditions, application, disease tissue cryopreservation conditions, etc. Recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome!
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. [] Cell line b :r
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cell lines for biological growth. Characteristics, source organ type, morphology, culture conditions, application system, disease tissue cryopreservation conditions, etc. Recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome!
|Rheumatoid arthritis synovial fibroblasts
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Cell line b :r
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines derived from American cell banks ( More than 3,000 types) Provide cell lines, organism growth characteristics, sources, organ types, morphology, culture conditions, application systems, disease tissue cryopreservation conditions, etc., recovery and cryopreservation instructions and information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture. No more hassle for your experiments!
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Cell lines b :r
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cell lines, organism growth characteristics, source organs Type, morphology, culture conditions, application, disease tissue cryopreservation conditions, etc. Recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome!
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[ ] Cell line b :r
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cell line organisms Growth characteristics, source, organ type, morphology, culture conditions, application system, disease tissue cryopreservation conditions, etc. Recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome!