HepaRG human liver cancer cell

Human liver cancer cells
(Human liver cancer cells)

[Cell number]

[Cell abbreviation]
Cells

[ Cell name]
(Human liver cancer cell)

[Background information]
See cell instructions

[Cell source]
Main source of cells , human liver cancer cells
, and a few well-known universities at home and abroad to establish departments

[Generations]

[Specifications]
Resuscitation culture bottle (one bottle ) or cryopreservation vials (two)

[Number of cells]
*

[Biosafety level]

[ Organism]
Human

[Tissue source]
Liver

[Cell morphology]
For details, please see the cell instructions section

【Cell Characteristics】
Adhesion

【Cell Viability】
)

【Cell Detection】
Cells do not contain , mycoplasma, bacteria, yeast and fungi

[Culture conditions]
See cell instructions for details

[Passaging method]
It is recommended to change the medium every two days

[Freezing conditions]
Please refer to the cell instructions for details

[Main literature]
Please refer to relevant published articles for details
After receipt Operating procedures and precautions
If the cells are adherent cells and are suspended or partially suspended when received, please centrifuge the suspended cells immediately and add % serum complete culture medium to a new culture dish/ The flask continues to be cultured for another day while the remaining adherent cells in the original culture flask are replaced with complete culture medium containing % serum for another day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem
. Adherent cells grow slowly and increase the serum concentration appropriately (the maximum cannot exceed %) , human liver cancer cells
According to the cell growth density, trypsin digestion may be considered and then transferred to a new culture bottle to continue culturing
. Uneven growth. If adherent cells are unevenly distributed, they will form an island shape. For growth, the cells can be digested, resuspended and dispersed, and fresh medium added for culture
Routine cell culture and passage procedures (please strictly follow aseptic operation)
Aspirate the medium from the original culture bottle , rinse the cells twice with buffer, and add. % trypsin to digest cells (pay attention to the digestion time, usually control it within)
Observe the digestion situation under a microscope. When the edges of the cells shrink and the adhesion becomes loose (it is not recommended to digest until the cells float), remove the trypsin and add it completely. culture medium, gently pipet the cell layer, try to blow off the cell layer, blow it loose
, transfer part of the cell suspension to a new culture dish/bottle, add appropriate complete culture medium, and stir the cell suspension. Evenly, culture in the incubator
, pay attention to the changes in the culture medium value, change the medium regularly (weekly), repeat the operation or freeze it after the cell density reaches %

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Human liver cancer cells

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Human liver cancer cells

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