CCD-18Co normal human colon tissue cells

Normal human colon tissue cells
(Normal human colon tissue cells)

[Cell number]

[Cell abbreviation]
Cells
< br/>[Cell name]
(Normal human colon tissue cells)

[Background information]
See cell instructions

[Cell source] < br/>The main source of cells, normal human colon tissue cells
, and a few well-known domestic and foreign universities established departments, age, month, gender, female, race, black

[Generation]

[Specifications]
Resuscitation culture bottle (one bottle) or cryopreservation vials (two)

[Number of cells]
*

【Biosafety Level】

【Organism】
Human

【Tissue Source】
Colon

【Cells Morphology]
Fibroblasts

[Cell characteristics]
Adhesion

[Cell viability]
)

[Cell Test]
The cells do not contain mycoplasma, bacteria, yeast and fungi

[Culture Conditions]
See the cell instructions for details

[ Passaging method]
It is recommended to change the medium every two days

[Freezing conditions]
See the cell instructions for details

[Main literature]
Please refer to relevant published articles for details
Operation procedures and precautions after receipt
If the cells are adherent cells and are suspended or partially suspended when received, please centrifuge the suspended cells immediately. Add % serum complete medium to the new culture dish/bottle and continue culturing for another day. At the same time, replace the remaining adherent cells in the original culture bottle with % serum complete medium for another day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, normal human colon tissue cells
Please contact the laboratory technician in time and they will follow up
Adherent cells grow slowly and appropriately Increase the serum concentration (the maximum cannot exceed %), or according to the cell growth density, consider trypsin digestion and then transfer to a new culture bottle to continue culturing
. If the adherent cells grow unevenly and are unevenly distributed, they will become To grow in an island shape, the cells can be digested, resuspended and dispersed, and added with fresh medium for culture
Routine cell culture and passage procedures (please strictly follow aseptic operations)
Aspirate out the original culture bottle Rinse the cells twice with culture medium and buffer, add .% trypsin to digest the cells (pay attention to the digestion time, usually control it at)
Observe the digestion situation under the microscope, when the edge of the cell shrinks and the adhesion loosens ( It is not recommended to digest until the cells are floating.) Remove trypsin, add complete culture medium, gently pipet the cell layer, try to blow off the cell layer, blow it loose
, transfer part of the cell suspension to a new culture dish/bottle , add appropriate complete culture medium, mix the cell suspension evenly, and culture it in the incubator
Pay attention to the changes in culture medium value, change the medium regularly (weekly), and repeat the operation after the cell density reaches % Or cryopreservation


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Normal human colon tissue cells


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The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks. , providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can Provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome

The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing Cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide cells We provide comprehensive technical support for cultivating normal human colon tissue cells, allowing you to experiment without any worries
Normal human colon tissue cells

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