HIEC normal human intestinal epithelial cells

Normal human intestinal epithelial cells
(Normal human intestinal epithelial cells)

[Cell number]

[Cell abbreviation]
Cells
< br/>[Cell name]
(Normal human intestinal epithelial cells)

[Background information]
See cell instructions

[Cell source] < br/>The main source of cells, normal human intestinal epithelial cells
, and a few well-known domestic and foreign universities established departments

[Generations]

[Specifications]
/>Resuscitation culture bottle (one bottle) or cryopreservation tube (two)

[Number of cells]
*

[Biosafety level] < br/>
[Organism]
Human

[Tissue source]
Small intestine

[Cell morphology]
Details See the cell instructions section

[Cell Characteristics]
Adhesion

[Cell Viability]
)

[Cell Detection 】
The cells do not contain mycoplasma, bacteria, yeast and fungi

[Cultivation conditions]
See the cell instructions for details

[Passaging method]
/>It is recommended to change the medium once every two days

[Freezing conditions]
Please refer to the cell instructions for details

[Main literature]
Please check the relevant publications for details Article
Operation procedures and precautions after receipt
If the cells are adherent cells or normal human intestinal epithelial cells
and are suspended or partially suspended when received, please transfer the suspended cells Centrifuge the cells immediately, add % serum complete medium to a new culture dish/bottle and continue culturing for another day. At the same time, the remaining adherent cells in the original culture flask are replaced with % serum complete medium and culture for another day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem
. Adherent cells grow slowly and increase the serum concentration appropriately (the maximum cannot exceed %) , or according to the cell growth density, trypsin digestion can be considered and then transferred to a new culture bottle to continue culturing
If the adherent cells grow unevenly and grow into islands, the cells can be digested , resuspend and disperse the cells, add fresh culture medium for culture
Routine cell culture and passage process (please strictly follow aseptic operation)
Aspirate the culture medium in the original culture bottle, and rinse both cells with buffer. times, add .% trypsin to digest the cells (pay attention to the digestion time, usually control it within)
Observe the digestion situation under a microscope, and remove the pancreas when the edges of the cells shrink and the cells become loose (it is not recommended to digest until the cells float) Enzyme, add complete culture medium, gently pipet the cell layer, try to blow off the cell layer, blow it away
Take part of the cell suspension and transfer it to a new culture dish/bottle, add appropriate complete culture medium, and Beat the cell suspension evenly and culture it in the incubator
Pay attention to the changes in the culture medium value, change the medium regularly (weekly), repeat the operation or freeze it after the cell density reaches %

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Normal human intestinal epithelial cells< br/>

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Normal human intestinal epithelial cells

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