HTR8-S/Vneo human chorionic trophoblast cells

/Human chorionic trophoblast cells
/(Human chorionic trophoblast cells)

[Cell number]

[Cell abbreviation]
/Cell

[Cell name]
/(Human chorionic trophoblast cells)

[Background information]
See cell instructions

[Cell source]
The main source of cells is: human chorionic trophoblast cells
, and a few famous domestic and foreign universities established in departments

[Generations]

[Specifications]
Resuscitation culture bottle (one bottle) or cryopreservation tube (two)

[Number of cells]
*
< br/>【Biosafety Level】

【Organism】
Human

【Tissue Source】
Trophoblast

【Cell morphology】
Epithelioid

【Cell characteristics】
Adhesion

【Cell viability】
)

【Cell Detection】
The cells do not contain mycoplasma, bacteria, yeast and fungi

【Cultivation Conditions】
See the cell instructions for details

[Passaging method]
It is recommended to change the medium every two days

[Freezing conditions]
See the cell instructions for details

[Main literature]
Please refer to relevant published articles for details
Operation procedures and precautions after receipt
If the cells are adherent cells and are suspended or partially suspended when received, please transfer the suspended cells immediately Centrifuge, add % serum complete medium to a new culture dish/bottle and continue culturing for another day. At the same time, the remaining adherent cells in the original culture bottle are replaced with % serum complete medium for another day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem
. Adherent cells grow slowly and increase the serum concentration appropriately (the maximum cannot exceed %) , or according to the cell growth density, trypsin digestion can be considered and then transferred to a new culture bottle to continue culturing
If the adherent cells grow unevenly and are unevenly distributed, human chorionic trophoblast cells will grow into islands.
The cells can be digested, resuspended and dispersed, and fresh medium added for culture
The routine cell culture and passage process (please strictly follow the aseptic operation)
Aspirate out the original culture bottle culture medium, rinse the cells twice with buffer, and add. % trypsin to digest cells (pay attention to the digestion time, usually control it within)
Observe the digestion situation under a microscope. When the edges of the cells shrink and the adhesion becomes loose (it is not recommended to digest until the cells float), remove the trypsin and add it completely. culture medium, gently pipet the cell layer, try to blow off the cell layer, blow it loose
, transfer part of the cell suspension to a new culture dish/bottle, add appropriate complete culture medium, and stir the cell suspension. Evenly, culture in the incubator
, pay attention to the changes in the culture medium value, change the medium regularly (weekly), repeat the operation or freeze storage after the cell density reaches %


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/人Chorionic trophoblast cells


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Instruction information, we have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free


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