HPDE6-C7 human normal pancreatic ductal epithelial cells

Human normal pancreatic ductal epithelial cells
(Human normal pancreatic ductal epithelial cells)

[Cell number]

[Cell abbreviation]
Cells

[Cell name]
(Human normal pancreatic ductal epithelial cells)

[Background information]
See cell instructions

[Cell Source]
The main source of cells is human normal pancreatic ductal epithelial cells
and a few well-known universities at home and abroad

[Generations]

[Specifications]
Resuscitation culture bottle (one bottle) or cryopreservation vial (two)

[Number of cells]
*

[Biosafety level 】

[Organism]
Human

[Tissue source]
Pancreas

[Cell morphology]
See the cell instructions section for details

[Cell Characteristics]
Adhesion

[Cell Viability]
)

[ Cell detection]
The cells do not contain mycoplasma, bacteria, yeast and fungi

[Culture conditions]
See the cell instructions for details

[Passaging method]
It is recommended to change the medium once every two days

[Freezing conditions]
See the cell instructions for details

[Main literature]
Please check for details Related published articles
Operation procedures and precautions after receipt
If the cells are adherent cells and are suspended or partially suspended when received, please centrifuge the suspended cells immediately and add % serum Transfer the complete medium to a new culture dish/bottle and continue culturing for another day. At the same time, the remaining adherent cells in the original culture flask are replaced with complete medium containing % serum for another day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem
. Adherent cells grow slowly and increase the serum concentration appropriately (the maximum cannot exceed %) , or according to the cell growth density, trypsin digestion can be considered and then transferred to a new culture bottle to continue culturing
If the adherent cells grow unevenly and are unevenly distributed, human normal pancreatic duct epithelial cells
To grow in an island shape, the cells can be digested, resuspended and dispersed, and then added with fresh medium for culture
The routine cell culture and passage process (please strictly follow the aseptic operation)
Aspirate out the original culture bottle The culture medium and buffer were used to rinse the cells twice, and .% trypsin was added to digest the cells (pay attention to the digestion time, usually controlled within)
. Observe the digestion situation under the microscope. When the edges of the cells shrink and the adhesion loosens, (It is not recommended to digest until the cells are floating.) Remove the trypsin, add complete culture medium, gently pipet the cell layer, try to blow off the cell layer, blow it loose
, transfer part of the cell suspension to a new culture dish/bottle , add appropriate complete culture medium, mix the cell suspension evenly, and culture it in the incubator
Pay attention to the changes in the culture medium value, change the medium regularly (weekly), and repeat the item after the cell density reaches % Operation or cryopreservation


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Human normal pancreatic ductal epithelial cells


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Human normal pancreatic ductal epithelial cells

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