MH7A<Rheumatoid arthritis synovial fibroblasts>

&;Rheumatoid arthritis synovial fibroblasts&;-b--:r
[Cell number]-b--:r

[Cell abbreviation]- b--:r
Cells
[Cell name]-b--:r
(Rheumatoid arthritis synovial fibroblasts)
[Background information]-b- -:r
&;The synoviocytes of patients with chronic indirect infection have been replaced by the synovial cells. -bに濜し-をproducedするという.
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[Cell source]-b--:r
The main sources of cells are,,,,r,,,RB,B,, and a few domestic and foreign Established in a famous university
[Generation]-b--:r
[Specifications]-b--:r
Resuscitation culture bottle (one bottle) or cryopreservation vial (two Branch)
[Number of cells]-b--:r
*()
[Biological safety level]-b--:r
[Organism]-b-- :r
Human
[Tissue source]-b--:r
Synovial membrane
[Cell morphology]-b--:r
Fibroblast-like< br/>【Cell Characteristics】-b--:r
Adhesion
【Cell Viability】-b--:r
% (bb B)
【Cell Detection】 -b--:r
The cells do not contain -, B, mycoplasma, bacteria, yeast and fungi
[Cultivation conditions] -b--:r
See the cell instructions for details
[Passaging method]-b--:r
Recommendation:-: Change the medium once every two days
[Freezing conditions]-b--:r
See the cell instructions for details
【Main Documents】-b--:r
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Cell Popularization of basic knowledge on cryopreservation and recovery:
Cell cryopreservation is one of the main methods of cell preservation. Using cryopreservation technology to store cells in -℃ liquid nitrogen at low temperature can temporarily remove the cells from the growth state and preserve their cellular characteristics, so that the cells can be revived for experiments when needed. Moreover, appropriately preserving a certain amount of cells can prevent cells from being lost due to contamination of the cultured cells or other accidents, and plays a role in cell seed preservation.
In addition, certain cells can also be purchased, donated, exchanged and transported in the form of cell cryopreservation. When cells are frozen, adding a protective agent to the culture medium - glycerol or dimethyl sulfoxide (final concentration %.%) can lower the freezing point of the solution. In addition, under slow freezing conditions, intracellular water leaks out, reducing Ice crystals form, thus preventing cell damage.
Using the "slow freezing and quick thawing" method can better ensure the survival of cells. The standard freezing speed starts from - to -℃/, and can be accelerated when the temperature is lower than -℃. After reaching -℃, it can be directly put into liquid nitrogen (-℃).
Steps of cell cryopreservation
() "Rheumatoid arthritis synovial fibroblasts" Select cells in the logarithmic growth phase, and it is best to change the medium one day before cryopreservation. Remove the cell culture medium from multiple culture bottles and digest with .% trypsin. Remove trypsin when appropriate and add a small amount of new culture medium. Use a pipette to absorb the culture medium and repeatedly blow the cells on the wall of the bottle to form a uniformly dispersed cell suspension. Suspension production cells do not need to be digested. The cells were then collected in centrifuge tubes and centrifuged (r/min).
() Remove the supernatant and add complete culture medium containing % calf serum. After pre-cooling at ℃ for 10 minutes, add sterile or glycerol drop by drop. Use a pipette to gently pipet the cells to make the cells even and the cell concentration It is between &;～&;/. (Does the preparation of cryopreservation culture medium have to use calf serum? The answer is not necessarily, it depends on the type of cultured cells.
() Dispense the above cell suspension into cryopreservation tubes , each tube... The lid of the cryopreservation tube should be tightly closed, and the cell name and cryopreservation date should be marked. At the same time, registration should be made (date, cell type and passage, number of cryopreservation tubes)
() Put the cryopreservation tubes containing cells into a freezing box and put them in a -℃ refrigerator overnight. It is best if there is a programmable cooler (put it in a -℃ refrigerator overnight and then put it into a liquid nitrogen tank); or it can be kept at ℃, and then transferred to to -°C,;-°C,; put into a liquid nitrogen tank.
() Cells frozen in liquid nitrogen can be stored for a long time, but for safety reasons, it is best to remove cells from one cryopreservation tube after half a year of cryopreservation. Resuscitate and culture, observe the growth, and then continue to freeze.
How to perform cell recovery:
. Take out the cryopreservation tube from the liquid nitrogen container, immerse it directly in ℃ warm water, and shake it from time to time to melt it as quickly as possible .
. Take out the cryovial from the ℃ water bath, open the lid, suck out the cell suspension with a pipette, add it to the centrifuge tube and add more than double the culture medium, mix;
. Centrifuge;
. Discard the supernatant, add culture medium containing % calf serum to resuspend the cells, count, adjust the cell density, inoculate the culture bottle, and culture in a ℃ incubator;
. Replace the culture medium the next day and continue culturing. .
Warm reminder
.Cultures previously cultured from the proliferation phase to the formation of dense monolayer cells can be used for cryopreservation, but it is best to use cells in the logarithmic growth phase. The best day before cryopreservation Change the culture medium once;
. When placing the cryovial into the liquid nitrogen container or taking it out, "rheumatoid arthritis synovial fibroblasts" should be protected (wear cotton gloves) to avoid frostbite. ;
. It is best to use newly prepared culture medium for cryopreservation and recovery.
When reviving cells, some first-time experimenters take the cryovials out of the liquid nitrogen and place them at room temperature. This often happens. The cryopreservation tube explodes. How to avoid this situation? Are there any details that should be paid attention to when tightening the cryopreservation tube?
The commonly used method is to take out the cryopreservation tube and use it in a clean workbench. Wipe the mouth of the test tube with an alcohol cotton ball, loosen the cap slightly, shake it in a water bath, transfer the cells to a test tube containing preheated culture medium, centrifuge quickly, and wash again with the culture medium.
R--b--:r
-Cell lines-b--:r
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-cell lines-b--:r
, company Specializing in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, Tissue, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome!
R--b--:r
-Cell lines-b--:r
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (three Thousands of species), providing cell line organisms, growth characteristics, sources, organs, < &;rheumatoid arthritis synovial fibroblasts&; types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions We have extensive experience in cell line culture and can provide all-round technical support for cell culture so that your experiments will be hassle-free!
R--b--:r
-Cell lines-b--:r
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (three Thousands of species), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
R--b--:r
-Cell lines-b--:r
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (three Thousands of species), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
R--b--:r
-Cell lines-b--:r
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (three Thousands of species), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
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-Cell line-b--:r
, < &;Rheumatoid arthritis synovial fibroblasts&; The company specializes in providing American cell banks Sources of normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, and cryopreservation conditions We have extensive experience in cell line culture and can provide all-round technical support for cell culture so that your experiments will be hassle-free!
R--b--:r
-Cell lines-b--:r
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (three Thousands of species), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!