MH7A rheumatoid arthritis synovial fibroblasts

Rheumatoid arthritis synovial fibroblasts
(Rheumatoid arthritis synovial fibroblasts)

[Cell number]

[Cell abbreviation]
Cells

[Cell Name]
(Rheumatoid Arthritis Synovial Fibroblasts)

[Background Information]
Patients with chronic indirect rheumatoid arthritis Synovial cells and rheumatoid arthritis synovial fibroblastsに濜しをproduces するという.

[Cell source]
The main source of cells and a few well-known domestic and foreign universities established departments

[Generation]

[Specifications]
Resuscitation culture bottle (one bottle) or cryopreservation vial (two)

[Number of cells]
*

[Biosafety level 】

【Organism】
Human

【Tissue source】
Synovial membrane

【Cell morphology】
Fibroblast-like

[Cell characteristics]
Adhesion

[Cell viability]
)

[ Cell detection]
The cells do not contain mycoplasma, bacteria, yeast and fungi

[Culture conditions]
See the cell instructions for details

[Passaging method]
It is recommended to change the medium once every two days

[Freezing conditions]
See cell instructions for details

[Main literature]
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Operation procedures and precautions after receiving
If the cells are adherent cells and received When in a suspended or partially suspended state, rheumatoid arthritis synovial fibroblasts
Please centrifuge the suspended cells immediately, add % serum complete culture medium to a new culture dish/bottle, and continue to culture for another day while keeping the original culture. The remaining adherent cells in the flask were replaced with complete culture medium containing % serum for 1 day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem
. Adherent cells grow slowly and increase the serum concentration appropriately (the maximum cannot exceed %) , or according to the cell growth density, trypsin digestion can be considered and then transferred to a new culture bottle to continue culturing
If the adherent cells grow unevenly and grow into islands, the cells can be digested , resuspend and disperse the cells, add fresh culture medium for culture
Routine cell culture and passage process (please strictly follow aseptic operation)
Aspirate the culture medium in the original culture bottle, and rinse both cells with buffer. times, add .% trypsin to digest the cells (pay attention to the digestion time, usually control it within)
Observe the digestion situation under a microscope, and remove the pancreas when the edges of the cells shrink and the cells become loose (it is not recommended to digest until the cells float) Enzyme, add complete culture medium, gently pipet the cell layer, try to blow off the cell layer, blow it away
Take part of the cell suspension and transfer it to a new culture dish/bottle, add appropriate complete culture medium, and Beat the cell suspension evenly and culture it in the incubator
Pay attention to the changes in the culture medium value, change the medium regularly (weekly), repeat the operation or freeze it after the cell density reaches %

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, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell line organisms, growth characteristics, sources, organs, types, morphologies, and culture conditions , applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free
< br/>, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell line organisms, growth characteristics, sources, organs, types, morphologies, culture conditions, and applications. , lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free

, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell line organisms, growth characteristics, sources, organs, types, morphologies, culture conditions, Application, line, disease, tissue, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide all-round technical support for cell culture, so that your experiments will be hassle-free
Rheumatoid Arthritis synovial fibroblasts

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The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell lines, organisms, Growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture technologies. Support allows you to experiment without any worries

The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell line organisms , growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture services. Technical support allows you to experiment without any worries

The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell lines, organisms, growth Characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide all-round technical support for cell culture. , allowing you to experiment without any worries

The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cell line organisms, growth characteristics, Source, organ, type, morphology, culture conditions, application, line, disease, tissue, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that Your experiment will no longer be troublesome


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The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell line organisms, growth characteristics, Source, organ, type, morphology, culture conditions, application, line, disease, tissue, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, allowing Your experiment will no longer be troublesome

The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the American cell bank, and provides cell line organisms and growth characteristics. , source, organ, type, morphology, culture conditions, application, line, disease, tissue, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture. Let your experiments be trouble-free

The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, and provides cell line organisms, growth characteristics, and sources. , organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, allowing you to Experiments are no longer troublesome

The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cell line organisms, growth characteristics, sources, and organs , type, morphology, culture conditions, application, line, disease, tissue, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, allowing you to experiment again. No hassle

, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell line organisms, growth characteristics, sources, organs, and types , morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free.


The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell line organisms, growth characteristics, sources, and organs. , type, morphology, culture conditions, application, line, disease, tissue, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, allowing you to experiment again. No hassle

, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell line organisms, growth characteristics, sources, organs, and types , morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free.

, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell line organisms, growth characteristics, sources, organs, types, and morphologies. , culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free

, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell line organisms, growth characteristics, sources, organs, and types , morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free.
Rheumatoid arthritis synovial fibroblasts

The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the US cell bank, providing cell lines Organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide comprehensive cell culture services. All-round technical support allows you to experiment without any worries

The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the American cell bank, and provides cell line organisms , growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture services. Technical support allows you to experiment without any worries

The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell lines, organisms, growth Characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide all-round technical support for cell culture. , allowing you to experiment without any trouble